Assessment of the Prevalence and Drug Susceptibility of Listeria monocytogenes Strains Isolated from Various Types of Meat
Abstract
:1. Introduction
2. Materials and Methods
2.1. Material
2.2. Isolation of L. monocytogenes Strains
2.3. Isolation of Genomic DNA
2.4. Species Identification
2.5. Evaluation of Pulsotypes Similarity (PFGE)
2.6. Molecular Serotyping of L. monocytogenes Strains
2.7. Drug Susceptibility Analysis
2.8. Statistical Analysis
3. Results
3.1. Evaluation of Pulsotypes Similarity (PFGE)
3.2. Drug Susceptibility Evaluation
3.3. L. monocytogenes Serotypes Determination and Distribution in Meat
4. Discussion
Author Contributions
Funding
Conflicts of Interest
References
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Profile Number | Drug-Resistance Profile | Number | ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
2016 | 2017 | 2018 | Total n = 120 | |||||||||||
Poultry n = 21 | Beef n = 14 | Pork n = 2 | Total in 2016 n = 37 | Poultry n = 27 | Beef n = 14 | Pork n = 7 | Total in 2017 n = 48 | Poultry n = 13 | Beef n = 11 | Pork n = 11 | Total in 2018 n = 35 | |||
I | R: - | 11 | 7 | 2 | 20 | 3 | 3 | 1 | 7 | 5 | 1 | 6 | 12 | 39 |
S: P, AM, MEM, E, SXT | (52.4%) | (50.0%) | (100.0%) | (54.1%) | (11.1%) | (21.4%) | (14.3%) | (14.6%) | (38.5%) | (9.0%) | (54.6%) | (32.4%) | (32.5%) | |
II | R: SXT | 5 | 1 | 0 | 6 | 4 | 0 | 1 | 5 | 0 | 2 | 1 | 3 | 14 |
S: P, AM, MEM, E | (23.8%) | (7.1%) | (0.0%) | (16.2%) | (11.1%) | (0.0%) | (14.3%) | (10.4%) | (0.0%) | (18.2%) | (9.1%) | (8.1%) | (11.7%) | |
III | R: P, AM, MEM, E, SXT, | 0 | 0 | 0 | 0 | 4 | 2 | 0 | 6 | 2 | 0 | 0 | 2 | 8 |
S: - | (0.0%) | (0.0%) | (0.0%) | (0.0%) | (11.1%) | (14.3%) | (0.0%) | (12.5%) | (15.4%) | (0.0%) | (0.0%) | (5.4%) | (6.7%) | |
IV | R: P, MEM, E, SXT | 1 | 0 | 0 | 1 | 1 | 0 | 0 | 1 | 2 | 3 | 0 | 5 | 7 |
S: AM | (4.8%) | (0.0%) | (0.0%) | (2.7%) | (3.7%) | (0.0%) | (0.0%) | (2.1%) | (15.4%) | (27.3%) | (0.0%) | (13.5%) | (5.8%) | |
V | R: MEM, E, SXT | 1 | 0 | 0 | 1 | 4 | 1 | 0 | 5 | 0 | 0 | 0 | 0 | 6 |
S: P, AM | (4.8%) | (0.0%) | (0.0%) | (2.7%) | (11.1%) | (7.1%) | (0.0%) | (10.4%) | (0.0%) | (0.0%) | (0.0%) | (0.0%) | (5.0%) | |
VI | R: MEM | 0 | 1 | 0 | 1 | 2 | 1 | 2 | 5 | 0 | 0 | 0 | 0 | 6 |
S: P, AM, E, SXT | (0.0%) | (7.1%) | (0.0%) | (2.7%) | (7.4%) | (7.1%) | (28.6%) | (10.4%) | (0.0%) | (0.0%) | (0.0%) | (0.0%) | (5.0%) | |
VII | R: AM, MEM, E, SXT | 0 | 1 | 0 | 1 | 2 | 0 | 1 | 3 | 0 | 1 | 0 | 1 | 5 |
S: P | (0.0%) | (7.1%) | (0.0%) | (2.7%) | (7.4%) | (0.0%) | (14.3%) | (6.3%) | (0.0%) | (9.1%) | (0.0%) | (2.7%) | (4.2%) | |
VIII | R: MEM, SXT | 0 | 0 | 0 | 0 | 2 | 2 | 0 | 4 | 0 | 0 | 1 | 1 | 5 |
S: P, AM, E | (0.0%) | (0.0%) | (0.0%) | (0.0%) | (7.4%) | (14.3%) | (0.0%) | (8.3%) | (0.0%) | (0.0%) | (9.1%) | (2.7%) | (4.2%) | |
IX | R: E, SXT | 1 | 2 | 0 | 3 | 1 | 1 | 0 | 2 | 0 | 0 | 0 | 0 | 5 |
S: P, AM, MEM | (4.8%) | (14.3%) | (0.0%) | (8.1%) | (3.7%) | (7.1%) | (0.0%) | (4.2%) | (0.0%) | (0.0%) | (0.0%) | (0.0%) | (4.2%) | |
X | R: MEM, E | 0 | 0 | 0 | 0 | 1 | 2 | 0 | 3 | 1 | 0 | 0 | 1 | 4 |
S: P, AM, SXT | (0.0%) | (0.0%) | (0.0%) | (0.0%) | (3.7%) | (14.3%) | (0.0%) | (6.3%) | (7.7%) | (0.0%) | (0.0%) | (2.7%) | (3.3%) | |
XI | R: P | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 3 | 3 |
S: AM, E, MEM, STX | (0.0%) | (0.0%) | (0.0%) | (0.0%) | (0.0%) | (0.0%) | (0.0%) | (0.0%) | (0.0%) | (18.2%) | (9.1%) | (8.1%) | (2.5%) | |
XII | R: AM, E, MEM | 0 | 1 | 0 | 1 | 1 | 0 | 1 | 2 | 0 | 0 | 0 | 0 | 3 |
S: P, SXT | (0.0%) | (7.1%) | (0.0%) | (2.7%) | (3.7%) | (0.0%) | (14.3%) | (4.2%) | (0.0%) | (0.0%) | (0.0%) | (0.0%) | (2.5%) | |
XIII | R: P, E, MEM | 1 | 0 | 0 | 1 | 1 | 0 | 1 | 2 | 0 | 0 | 0 | 0 | 3 |
S: AM, SXT | (4.8%) | (0.0%) | (0.0%) | (2.7%) | (3.7%) | (0.0%) | (14.3%) | (4.2%) | (0.0%) | (0.0%) | (0.0%) | (0.0%) | (2.5%) | |
XIV | R: P, E, SXT | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 2 | 2 |
S: AM, MEM | (0.0%) | (0.0%) | (0.0%) | (0.0%) | (0.0%) | (0.0%) | (0.0%) | (0.0%) | (7.7%) | (0.0%) | (9.1%) | (5.4%) | (1.7%) | |
XV | R: E | 1 | 0 | 0 | 1 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 2 |
S: P, AM, MEM, STX | (4.8%) | (0.0%) | (0.0%) | (2.7%) | (0.0%) | (7.1%) | (0.0%) | (2.1%) | (0.0%) | (0.0%) | (0.0%) | (0.0%) | (1.7%) | |
XVI | R: P, MEM | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 0 | 1 | 2 |
S: AM, E, SXT | (0.0%) | (0.0%) | (0.0%) | (0.0%) | (0.0%) | (0.0%) | (14.3%) | (2.1%) | (7.7%) | (0.0%) | (0.0%) | (2.7%) | (1.7%) | |
XVII | R: P, AM, E, MEM | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
S: SXT | (0.0%) | (7.1%) | (0.0%) | (2.7%) | (0.0%) | (0.0%) | (0.0%) | (0.0%) | (0.0%) | (0.0%) | (0.0%) | (0.0%) | (0.8%) | |
XVIII | R: P, AM, E | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 1 |
S: MEM, SXT | (0.0%) | (0.0%) | (0.0%) | (0.0%) | (0.0%) | (0.0%) | (0.0%) | (0.0%) | (7.8%) | (0.0%) | (0.0%) | (2.7%) | (0.8%) | |
XIX | R: P, AM, E, SXT | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 1 |
S: MEM | (0.0%) | (0.0%) | (0.0%) | (0.0%) | (0.0%) | (0.0%) | (0.0%) | (0.0%) | (0.0%) | (9.0%) | (0.0%) | (2.7%) | (0.8%) | |
XX | R: P, MEM, SXT | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 1 |
S: AM, E | (0.0%) | (0.0%) | (0.0%) | (0.0%) | (0.0%) | (0.0%) | (0.0%) | (0.0%) | (0.0%) | (9.0%) | (0.0%) | (2.7%) | (0.8%) | |
XXI | R: P, AM, SXT | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 |
S: MEM, E | (0.0%) | (0.0%) | (0.0%) | (0.0%) | (0.0%) | (0.0%) | (0.0%) | (0.0%) | (0.0%) | (0.0%) | (9.1%) | (2.7%) | (0.8%) | |
XXII | R: P, AM, MEM, SXT | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 |
S: E | (0.0%) | (0.0%) | (0.0%) | (0.0%) | (3.7%) | (0.0%) | (0.0%) | (2.1%) | (0.0%) | (0.0%) | (0.0%) | (0.0%) | (0.8%) |
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Skowron, K.; Wałecka-Zacharska, E.; Wiktorczyk-Kapischke, N.; Skowron, K.J.; Grudlewska-Buda, K.; Bauza-Kaszewska, J.; Bernaciak, Z.; Borkowski, M.; Gospodarek-Komkowska, E. Assessment of the Prevalence and Drug Susceptibility of Listeria monocytogenes Strains Isolated from Various Types of Meat. Foods 2020, 9, 1293. https://doi.org/10.3390/foods9091293
Skowron K, Wałecka-Zacharska E, Wiktorczyk-Kapischke N, Skowron KJ, Grudlewska-Buda K, Bauza-Kaszewska J, Bernaciak Z, Borkowski M, Gospodarek-Komkowska E. Assessment of the Prevalence and Drug Susceptibility of Listeria monocytogenes Strains Isolated from Various Types of Meat. Foods. 2020; 9(9):1293. https://doi.org/10.3390/foods9091293
Chicago/Turabian StyleSkowron, Krzysztof, Ewa Wałecka-Zacharska, Natalia Wiktorczyk-Kapischke, Karolina Jadwiga Skowron, Katarzyna Grudlewska-Buda, Justyna Bauza-Kaszewska, Zuzanna Bernaciak, Miłosz Borkowski, and Eugenia Gospodarek-Komkowska. 2020. "Assessment of the Prevalence and Drug Susceptibility of Listeria monocytogenes Strains Isolated from Various Types of Meat" Foods 9, no. 9: 1293. https://doi.org/10.3390/foods9091293