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Open AccessArticle

Packaging Design Using Mustard Seeds as a Natural Antimicrobial: A Study on Inhibition of Pseudomonas fragi in Liquid Medium

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Food Quality and Design Group, Wageningen University and Research, P.O. Box 17, 6700 AA Wageningen, The Netherlands
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Agriculture and Forestry Faculty, Universitas Sulawesi Barat, Majene 91412, Indonesia
*
Author to whom correspondence should be addressed.
Foods 2020, 9(6), 789; https://doi.org/10.3390/foods9060789
Received: 13 May 2020 / Revised: 3 June 2020 / Accepted: 10 June 2020 / Published: 16 June 2020
Pseudomonas fragi is the dominant spoilage organism in various foods, especially in spoiled milk, fish, and meats. Its growth can be inhibited by releasing allyl isothiocyanate (AITC) from ground mustard seeds in food packages. This paper aims to investigate the antimicrobial potential of ground mustard seeds against P. fragi growth and the effectiveness of released AITC concentration from mustard seeds on microbial inhibition of the spoilage bacteria growing in the liquid medium. The AITC concentration in the headspace and the liquid medium was measured and the growth of P. fragi in the liquid medium was monitored. Depending on the concentration of AITC, not only growth was inhibited but a reduction of the total count of P. fragi was even observed. The inactivation rate (k) of P. fragi was estimated using first-order inactivation kinetics and the minimum gaseous-released AITC to inactivate P. fragi was determined. Higher AITC concentration in the headspace and liquid medium was observed when using a higher amount of ground mustard seeds and a lower food to headspace ratio. Increasing the amount of ground mustard seeds (>100 mg per 10 mL liquid medium) led to full inactivation of P. fragi in 48 hours. By using an inhibition sigmoid Emax model, the minimum gaseous-released AITC for inactivation of P. fragi in 48 hours was observed around 15 µg/L headspace. These results indicate that inhibition of the spoilage bacteria and extending the shelf life using ground mustard seeds is only possible by applying a careful design of the packaging system. View Full-Text
Keywords: mustard seeds; allyl isothiocyanate; Pseudomonas fragi; spoilage bacteria; microbial inhibition; shelf life; antimicrobial packaging mustard seeds; allyl isothiocyanate; Pseudomonas fragi; spoilage bacteria; microbial inhibition; shelf life; antimicrobial packaging
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Bahmid, N.A.; Heising, J.; Fogliano, V.; Dekker, M. Packaging Design Using Mustard Seeds as a Natural Antimicrobial: A Study on Inhibition of Pseudomonas fragi in Liquid Medium. Foods 2020, 9, 789.

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