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Open AccessArticle

Purification and Characterization of Pathogenesis Related Class 10 Panallergens

US Department of Agriculture, Agricultural Research Service, Southern Regional Research Center, New Orleans, LA 70124, USA
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Foods 2019, 8(12), 609; https://doi.org/10.3390/foods8120609
Received: 24 September 2019 / Revised: 8 November 2019 / Accepted: 20 November 2019 / Published: 22 November 2019
Oral allergy syndrome (OAS) describes an allergic reaction where an individual sensitized by pollen allergens develops symptoms after eating certain foods. OAS is caused by cross-reactivity among a class of proteins ubiquitous in plants called pathogenesis related class 10 (PR-10) proteins. The best characterized PR-10 protein is Bet v 1 from birch pollen and its putative function is binding hydrophobic ligands. We cloned a subset of seven recombinant PR-10 proteins from pollens, peanuts, and hazelnuts and developed a standard purification method for them. Immunoglobulin E (IgE) binding of purified PR-10 proteins was analyzed by ImmunoCAP ISAC microarray and enzyme-linked immunosorbent assays (ELISAs) with sera from allergic patients. We investigated the binding activities of PR10s by testing 16 different ligands with each protein and compared their secondary structures using circular dichroism (CD). The PR-10s in this study had very similar CD spectra, but bound IgE with very different affinities. All seven proteins showed a similar pattern of binding to the polyphenol ligands (resveratrol, flavonoids, and isoflavones) and variable binding to other potential ligands (fatty acids, sterols, and plant hormones). We suggest our protocol has the potential to be a near-universal method for PR-10 purification that will facilitate further research into this important class of panallergens.
Keywords: panallergen; pollen; peanut; hazelnut; protein purification; structure; ligand binding; IgE binding; flavonoids panallergen; pollen; peanut; hazelnut; protein purification; structure; ligand binding; IgE binding; flavonoids
MDPI and ACS Style

McBride, J.K.; Cheng, H.; Maleki, S.J.; Hurlburt, B.K. Purification and Characterization of Pathogenesis Related Class 10 Panallergens. Foods 2019, 8, 609.

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