Lactic Acid Bacteria from Kefir Increase Cytotoxicity of Natural Killer Cells to Tumor Cells
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials
2.2. Cell Culture
2.3. Preparation of Lactic Acid Bacteria Mixture
2.4. Measurement of NK Cell Activity and Tumor Cytotoxicity
2.5. RT-PCR (Reverse Transcription Polymerase Chain Reaction)
2.6. IFN-γ Measurement
2.7. Statistical Analysis
3. Results and Discussion
3.1. Induction of NK Activity by the Six Lactic Acid Bacteria from Homemade-Kefir
3.2. Induction of Cytotoxicity by the Six Lactic Acid Bacteria Against Human Colorectal Tumor Cells
3.3. Induction of Cytotoxicity of KHYG-1 Cells by the Killed Lactic Acid Bacteria Mixture
3.4. Increased Expression and Secretion Levels of IFN-γ in KHYG-1 Cells
4. Conclusions
Author Contributions
Conflicts of Interest
References
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Strain | Primer Sequence | PCR Condition | Ref. | |
---|---|---|---|---|
L. lactis subsp. Lactis | sense | 5′-CGTTATGGATTTGATGGATATAAAGC-3′ | 94 °C 9 min, 94 °C 30 s, 50 °C 30 s, 72 °C 60 s × 45 cycles, 72 °C 7 min | [27] |
antisense | 5′-ACTCTTCTTAAGAACAAGTTTAACAGC-3′ | |||
L. lactis subsp. Cremoris | sense | 5′-CGTTATGGATTTGATGGATATAAAGC-3′ | 94 °C 9 min, 94 °C 30 s, 50 °C 30 s, 72 °C 60 s × 45 cycles, 72 °C 7 min | |
antisense | 5′-ACTCTTCTTAAGAACAAGTTTAACAGC-3′ | |||
L. lactis subsp. lactis biovar diacetylactis | sense | 5′-CTTCGTTATGATTTTACA-3′ | 94 °C 30 s, 94 °C 30 s, 46 °C 60 s, 72 °C 60 s × 30 cycles, 72 °C 2 min | [28,29] |
antisense | 5′-AATATCAACAATTCCATG-3′ | |||
Leuconostoc mesenteroides subsp. Cremoris (Genus-specific primers for Leuconostoc) | sense | 5′-AACTTAGTGTCGCATGAC-3′ | 94 °C 5 min, 94 °C 60 s, 60 °C 60 s, 72 °C 60 s × 30 cycles, 72 °C 10 min | [30] |
antisense | 5′-AGTCGAGTTACAGACTACAA-3′ | |||
Lactobacillus plantarum | sense | 5′-CCGTTTATGCGGAACACCTA-3′ | 94 °C 3 min, 94 °C 30 s, 54 °C 10 s, 72 °C 30 s × 30 cycles, 72 °C 5 min | [31] |
antisense | 5′-TCGGGATTACCAAACATCAC-3′ | |||
Lactobacillus casei | sense | 5-TGCACTGAGATTCGACTTAA-3 | 94 °C 3 min, 94 °C 45 s, 45 °C 45 s, 72 °C 60 s × 30 cycles, 72 °C 5 min | [32] |
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Yamane, T.; Sakamoto, T.; Nakagaki, T.; Nakano, Y. Lactic Acid Bacteria from Kefir Increase Cytotoxicity of Natural Killer Cells to Tumor Cells. Foods 2018, 7, 48. https://doi.org/10.3390/foods7040048
Yamane T, Sakamoto T, Nakagaki T, Nakano Y. Lactic Acid Bacteria from Kefir Increase Cytotoxicity of Natural Killer Cells to Tumor Cells. Foods. 2018; 7(4):48. https://doi.org/10.3390/foods7040048
Chicago/Turabian StyleYamane, Takuya, Tatsuji Sakamoto, Takenori Nakagaki, and Yoshihisa Nakano. 2018. "Lactic Acid Bacteria from Kefir Increase Cytotoxicity of Natural Killer Cells to Tumor Cells" Foods 7, no. 4: 48. https://doi.org/10.3390/foods7040048