Biosensors for the Detection of Food Pathogens
AbstractFood pathogens frequently cause foodborne diseases. There is a need to rapidly identify the source of the bacteria in order to contain their spread and epidemics. A pre-enrichment culture or a direct culture on agar plate are standard microbiological methods. In this review, we present an update on alternative molecular methods to nucleic acid-based detection for species identification. Biosensor-based methods rely on the recognition of antigen targets or receptors by antibodies, aptamers or high-affinity ligands. The captured antigens may be then directly or indirectly detected through an antibody or high-affinity and high-specificity recognition molecule. Various different detection methods are discussed, from label-free sensors and immunosensors to fluorescence-based ones. Each method shows advantages and disadvantages in terms of equipment, sensitivity, simplicity and cost-effectiveness. Finally, lab-on-a-chip (LOC) devices are introduced briefly, with the potential to be fast, sensitive and useful for on-site bacteria detection in food processing laboratories to check potential contamination by sample monitoring combined with a rapid pre-enrichment step. View Full-Text
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Poltronieri, P.; Mezzolla, V.; Primiceri, E.; Maruccio, G. Biosensors for the Detection of Food Pathogens. Foods 2014, 3, 511-526.
Poltronieri P, Mezzolla V, Primiceri E, Maruccio G. Biosensors for the Detection of Food Pathogens. Foods. 2014; 3(3):511-526.Chicago/Turabian Style
Poltronieri, Palmiro; Mezzolla, Valeria; Primiceri, Elisabetta; Maruccio, Giuseppe. 2014. "Biosensors for the Detection of Food Pathogens." Foods 3, no. 3: 511-526.