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Article

Impact of Pretreatment Methods on Yield and Composition of Cold-Pressed Black Cumin (Nigella sativa L.) Seed Oil

by
Valdas Laukagalis
1,
Živilė Tarasevičienė
1,*,
Mindaugas Visockis
2,
Kiril Kazancev
3,
Eglė Sendžikienė
3,
Anna Kiełtyka-Dadasiewicz
4,
Saulius Šatkauskas
2 and
Aurelija Paulauskienė
1
1
Department of Plant Biology and Food Sciences, Faculty of Agronomy, Agriculture Academy, Vytautas Magnus University, Donelaičio Str. 58, 44248 Kaunas, Lithuania
2
Department of Biochemistry, Research Institute of Nature and Technology Sciences, Faculty of Natural Sciences, Vytautas Magnus University, Universiteto Str. 10-506, 53361 Akademija, Lithuania
3
Department of Environment and Ecology, Faculty of ForestSciences and Ecology, Agriculture Academy, Vytautas Magnus University, Donelaičio Str. 58, 44248 Kaunas, Lithuania
4
Department of Plant Production Technology and Commodity Science, University of Life Sciences in Lublin, Akademicka 13, 20-950 Lublin, Poland
*
Author to whom correspondence should be addressed.
Foods 2025, 14(24), 4234; https://doi.org/10.3390/foods14244234
Submission received: 4 October 2025 / Revised: 14 November 2025 / Accepted: 6 December 2025 / Published: 9 December 2025

Abstract

This study aimed to evaluate the effects of different pretreatment methods on the yield and chemical composition of Nigella sativa L. (i.e., black cumin) cold-pressed oil. Different pretreatment methods used included convection heating, microwave, ultrasound and pulsed electric fields (i.e., PEF); we investigated their effects on key parameters, including oil yield, fatty acid composition, total phenol and flavonoid content, peroxide value, acidity and antioxidant activity. However, no single pretreatment method was found to be better than all others. Instead, ultrasonication and pulsed electric fields (PEF) showed significant advantages. Ultrasonication showed the highest total phenolic content and improved oxidative stability, while PEF improved flavonoid content and antioxidant activity. Therefore, these findings suggest that a combination of several pretreatment options should be considered depending on specific industrial goals related to quality improvement and sustainability. This research also contributes to the existing knowledge on Nigella sativa L. oil processing and provides additional insights for optimizing extraction technique.
Keywords: oil extraction optimization; antioxidants; fatty acids; phenolic content; ultrasonication; pulsed electric fields oil extraction optimization; antioxidants; fatty acids; phenolic content; ultrasonication; pulsed electric fields

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MDPI and ACS Style

Laukagalis, V.; Tarasevičienė, Ž.; Visockis, M.; Kazancev, K.; Sendžikienė, E.; Kiełtyka-Dadasiewicz, A.; Šatkauskas, S.; Paulauskienė, A. Impact of Pretreatment Methods on Yield and Composition of Cold-Pressed Black Cumin (Nigella sativa L.) Seed Oil. Foods 2025, 14, 4234. https://doi.org/10.3390/foods14244234

AMA Style

Laukagalis V, Tarasevičienė Ž, Visockis M, Kazancev K, Sendžikienė E, Kiełtyka-Dadasiewicz A, Šatkauskas S, Paulauskienė A. Impact of Pretreatment Methods on Yield and Composition of Cold-Pressed Black Cumin (Nigella sativa L.) Seed Oil. Foods. 2025; 14(24):4234. https://doi.org/10.3390/foods14244234

Chicago/Turabian Style

Laukagalis, Valdas, Živilė Tarasevičienė, Mindaugas Visockis, Kiril Kazancev, Eglė Sendžikienė, Anna Kiełtyka-Dadasiewicz, Saulius Šatkauskas, and Aurelija Paulauskienė. 2025. "Impact of Pretreatment Methods on Yield and Composition of Cold-Pressed Black Cumin (Nigella sativa L.) Seed Oil" Foods 14, no. 24: 4234. https://doi.org/10.3390/foods14244234

APA Style

Laukagalis, V., Tarasevičienė, Ž., Visockis, M., Kazancev, K., Sendžikienė, E., Kiełtyka-Dadasiewicz, A., Šatkauskas, S., & Paulauskienė, A. (2025). Impact of Pretreatment Methods on Yield and Composition of Cold-Pressed Black Cumin (Nigella sativa L.) Seed Oil. Foods, 14(24), 4234. https://doi.org/10.3390/foods14244234

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