Fungal Biostarter Effect on the Quality of Dry-Aged Beef
Abstract
:1. Introduction
2. Materials and Methods
2.1. Research Material
2.1.1. Sample Collection and Preparation
2.1.2. Fungal Biostarter
2.2. Research Methods
2.2.1. Aging Conditions
2.2.2. The pH Determination
2.2.3. Color Measurement
2.2.4. Glucose and Lactate Concentration in Tissue, and Muscle Glycolytic Potential
2.2.5. Water-Holding Capacity and Meat Plasticity
2.2.6. Meat Composition
2.2.7. Determination of the Shear Force
2.2.8. The Content of Malondialdehyde
2.2.9. Sodium Dodecyl Sulfate–Polyacrylamide Gel Electrophoresis (SDS-PAGE)
2.2.10. Consumer Evaluation of the Sensory Quality of Dry-Aged Beef
2.2.11. Fungal Biostarter Taxonomic Identification
2.2.12. Bacterial DNA Extraction
2.2.13. Bacterial Metabarcoding
2.2.14. Statistical Analysis
3. Results
3.1. Physicochemical Changes of DAB
3.2. Myofibrillar Proteins Profiles of DAB
3.3. Changes in Sensory Quality of Dry-Aged Beef
3.4. Bacterial Diversity of DAB
4. Discussion
5. Conclusions
6. Patents
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Traits | Group | SEM | |
---|---|---|---|
Biostarter− | Biostarter+ | ||
pH | 5.61 | 5.60 | 0.05 |
Color parameters: L* | 33.03 | 34.11 | 1.93 |
a* | 19.32 | 19.74 | 1.69 |
b* | 16.50 | 16.27 | 1.64 |
Glucose (mmol/L) | 5.83 | 5.29 | 0.70 |
Lactate (mmol/L) | 57.24 | 63.81 | 5.98 |
Glycolytic potential (mmol/L) | 68.90 | 74.40 | 6.75 |
WHC (cm2/g) | 13.49 | 14.00 | 1.68 |
Plasticity (cm2) | 3.37 | 3.30 | 0.21 |
Moisture content (%) | 66.84 | 68.97 | 1.41 |
Fat content (%) | 9.16 | 8.80 | 1.38 |
Protein content (%) | 21.61 | 20.97 | 0.51 |
Connective tissue (%) | 0.87 | 0.84 | 0.14 |
Ash content (%) | 0.66 | 0.90 | 0.14 |
Traits | Group | SEM | |
---|---|---|---|
Biostarter− | Biostarter+ | ||
pH | 5.75 | 5.84 | 0.07 |
Color parameters: L* | 28.76 | 26.63 | 1.57 |
a* | 25.34 | 24.61 | 1.72 |
b* | 24.38 | 22.67 | 1.67 |
Shear force after grilling (N) | 75.35 | 65.91 | 4.27 |
Penetration force after grilling (mm) | 12.82 | 12.11 | 0.55 |
The content of malondialdehyde (mg/kg) | 0.98 | 0.52 | 0.43 |
Measure | Biostarter− (Sample 1) | Biostarter− (Sample 2) | Biostarter+ (Sample 1) | Biostarter+ (Sample 2) |
---|---|---|---|---|
Chao1 | 14 | 17 | 10 | 10 |
Shannon | 0.93 | 2.25 | 1.21 | 1.23 |
Simpson | 0.37 | 0.86 | 0.59 | 0.61 |
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Przybylski, W.; Jaworska, D.; Płecha, M.; Dukaczewska, K.; Ostrowski, G.; Sałek, P.; Sawicki, K.; Pawłowska, J. Fungal Biostarter Effect on the Quality of Dry-Aged Beef. Foods 2023, 12, 1330. https://doi.org/10.3390/foods12061330
Przybylski W, Jaworska D, Płecha M, Dukaczewska K, Ostrowski G, Sałek P, Sawicki K, Pawłowska J. Fungal Biostarter Effect on the Quality of Dry-Aged Beef. Foods. 2023; 12(6):1330. https://doi.org/10.3390/foods12061330
Chicago/Turabian StylePrzybylski, Wiesław, Danuta Jaworska, Magdalena Płecha, Karina Dukaczewska, Grzegorz Ostrowski, Piotr Sałek, Krzysztof Sawicki, and Julia Pawłowska. 2023. "Fungal Biostarter Effect on the Quality of Dry-Aged Beef" Foods 12, no. 6: 1330. https://doi.org/10.3390/foods12061330