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Article

A Molecular Toolbox to Identify and Quantify Grape Varieties: On the Trace of “Glera”

by
Ilaria Carrara
1,
Valeria Terzi
1,*,
Roberta Ghizzoni
1,
Stefano Delbono
1,
Giorgio Tumino
2,
Manna Crespan
3,
Massimo Gardiman
3,
Enrico Francia
4 and
Caterina Morcia
1
1
Consiglio per la Ricerca in Agricoltura e l’Analisi dell’Economia Agraria, Centro di Ricerca Genomica e Bioinformatica (CREA-GB), Via San Protaso 302, 29017 Fiorenzuola d’Arda, Italy
2
Plant Breeding, Wageningen University and Research, Droevendaalsesteeg 1, 6708 PB Wageningen, The Netherlands
3
Consiglio per la Ricerca in Agricoltura e l’Analisi dell’Economia Agraria, Centro di Ricerca Viticoltura ed Enologia (CREA-VE), Viale 28 Aprile 26, 31015 Conegliano, Italy
4
Department of Life Science, Centre BIOGEST-SITEIA, University of Study of Modena and Reggio Emilia, Via Amendola, n. 2, 42122 Reggio Emilia, Italy
*
Author to whom correspondence should be addressed.
Foods 2023, 12(16), 3091; https://doi.org/10.3390/foods12163091
Submission received: 13 July 2023 / Revised: 11 August 2023 / Accepted: 15 August 2023 / Published: 17 August 2023
(This article belongs to the Special Issue Worldwide Inquiries into Natural Eternity—Wine: Chemical Composition, Stability, Health Benefits, and Authenticity Issues)
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Abstract

A pillar of wine authenticity is the variety/ies used. Ampelographic descriptors and SSR markers, included in several national and international databases, are extensively used for varietal identification purposes. Recently, SNP markers have been proposed as useful for grape varietal identification and traceability. Our study has been directed toward the development of a molecular toolbox able to track grape varieties from the nursery to the must. Two complementary approaches were developed, exploiting SNP markers with two different technologies, i.e., a high-throughput platform for varietal identification and a digital PCR system for varietal quantification. As proof-of-concept, the toolbox was successfully applied to the identification and quantification of the “Glera” variety along the Prosecco wine production chain. The assays developed found their limits in commercial, aged wines.
Keywords: chip digital PCR; high-throughput SNP genotyping; varietal traceability; grape chain authenticity chip digital PCR; high-throughput SNP genotyping; varietal traceability; grape chain authenticity

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MDPI and ACS Style

Carrara, I.; Terzi, V.; Ghizzoni, R.; Delbono, S.; Tumino, G.; Crespan, M.; Gardiman, M.; Francia, E.; Morcia, C. A Molecular Toolbox to Identify and Quantify Grape Varieties: On the Trace of “Glera”. Foods 2023, 12, 3091. https://doi.org/10.3390/foods12163091

AMA Style

Carrara I, Terzi V, Ghizzoni R, Delbono S, Tumino G, Crespan M, Gardiman M, Francia E, Morcia C. A Molecular Toolbox to Identify and Quantify Grape Varieties: On the Trace of “Glera”. Foods. 2023; 12(16):3091. https://doi.org/10.3390/foods12163091

Chicago/Turabian Style

Carrara, Ilaria, Valeria Terzi, Roberta Ghizzoni, Stefano Delbono, Giorgio Tumino, Manna Crespan, Massimo Gardiman, Enrico Francia, and Caterina Morcia. 2023. "A Molecular Toolbox to Identify and Quantify Grape Varieties: On the Trace of “Glera”" Foods 12, no. 16: 3091. https://doi.org/10.3390/foods12163091

APA Style

Carrara, I., Terzi, V., Ghizzoni, R., Delbono, S., Tumino, G., Crespan, M., Gardiman, M., Francia, E., & Morcia, C. (2023). A Molecular Toolbox to Identify and Quantify Grape Varieties: On the Trace of “Glera”. Foods, 12(16), 3091. https://doi.org/10.3390/foods12163091

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