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Article

An In Vitro and In Vivo Translational Research Approach for the Assessment of Sensitization Capacity and Residual Allergenicity of an Extensive Whey Hydrolysate for Cow’s Milk-Allergic Infants

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Danone Nutricia Research, 3584 CT Utrecht, The Netherlands
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Utrecht Institute for Pharmaceutical Sciences, Faculty of Science, Utrecht University, 3584 CS Utrecht, The Netherlands
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Polpharma Biologics B.V., 3584 CM Utrecht, The Netherlands
*
Author to whom correspondence should be addressed.
Academic Editors: Linda Monaci and Elisabetta De Angelis
Foods 2022, 11(14), 2005; https://doi.org/10.3390/foods11142005
Received: 8 June 2022 / Revised: 30 June 2022 / Accepted: 5 July 2022 / Published: 7 July 2022
Introduction: Hypoallergenic formulas prepared from hydrolyzed cow’s milk proteins are often used for the management of cow’s milk allergy (CMA) in infants. In this study, both in vitro assays and an in vivo mouse model for CMA were used to assess the sensitizing and allergenic potential of a newly developed, extensive whey hydrolysate (eWH). Methods: Gel permeation chromatography was used to characterize the molecular weight distribution of the peptides. Residual antigenicity was measured using a beta-lactoglobulin ELISA as well as with immunoblotting using anti-beta-lactoglobulin (BLG) and anti-alpha-lactalbumin antibodies. In vitro residual allergenicity was assessed using huFcεRIα-RBL-2H3 cells sensitized with anti-bovine BLG human IgE. In vivo sensitizing and allergenic potential was assessed in a CMA mouse model by measuring the acute allergic skin response, anaphylactic shock score, body temperature, serum mMCP-1, whey-specific IgE, and cytokines. Results: There was no in vitro residual antigenicity and allergenicity observed of the eWH. Mice sensitized with eWH showed no acute allergic skin reaction after challenge with whey, confirmed by an absence of whey-specific IgE and anaphylactic symptoms and decrease in body temperature and mMCP-1 levels. Conclusions: Results from our in vitro and in vivo translational approach to assess sensitization capacity and residual allergenicity indicate that the newly developed eWH is safe for use in CMA infants. This was subsequently confirmed in a clinical study in which this eWH was tolerated by more than 90% (with 95% confidence) of infants or children with confirmed CMA. View Full-Text
Keywords: RBL; beta-lactoglobulin; whey hydrolysates; chimeric; monoclonal antibodies; IgE RBL; beta-lactoglobulin; whey hydrolysates; chimeric; monoclonal antibodies; IgE
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MDPI and ACS Style

Knipping, K.; Buelens, L.; Simons, P.J.; Garssen, J. An In Vitro and In Vivo Translational Research Approach for the Assessment of Sensitization Capacity and Residual Allergenicity of an Extensive Whey Hydrolysate for Cow’s Milk-Allergic Infants. Foods 2022, 11, 2005. https://doi.org/10.3390/foods11142005

AMA Style

Knipping K, Buelens L, Simons PJ, Garssen J. An In Vitro and In Vivo Translational Research Approach for the Assessment of Sensitization Capacity and Residual Allergenicity of an Extensive Whey Hydrolysate for Cow’s Milk-Allergic Infants. Foods. 2022; 11(14):2005. https://doi.org/10.3390/foods11142005

Chicago/Turabian Style

Knipping, Karen, Laura Buelens, Peter J. Simons, and Johan Garssen. 2022. "An In Vitro and In Vivo Translational Research Approach for the Assessment of Sensitization Capacity and Residual Allergenicity of an Extensive Whey Hydrolysate for Cow’s Milk-Allergic Infants" Foods 11, no. 14: 2005. https://doi.org/10.3390/foods11142005

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