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Article

Autofluorescence Detection Method for Dental Plaque Bacteria Detection and Classification: Example of Porphyromonas gingivalis, Aggregatibacter actinomycetemcomitans, and Streptococcus mutans

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Institute of Electrical and Control Engineering, National Yang Ming Chiao Tung University, 1001 University Road, Hsinchu City 30010, Taiwan
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Department of Electrical and Computer Engineering, National Yang Ming Chiao Tung University, 1001 University Road, Hsinchu City 30010, Taiwan
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Department of Laboratory Medicine, National Taiwan University Hospital Hsin-Chu Branch, No. 25 Ln. 442 Jingguo Road, Hsinchu City 30010, Taiwan
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Institute of Molecular Medicine and Biochemical Engineering, National Yang Ming Chiao Tung University, 1001 University Road, Hsinchu City 30010, Taiwan
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Institute of Oral Biology, National Yang Ming Chiao Tung University, 155 Linong Street, Taipei City 11221, Taiwan
*
Author to whom correspondence should be addressed.
Academic Editors: Liviu Steier and Gianrico Spagnuolo
Dent. J. 2021, 9(7), 74; https://doi.org/10.3390/dj9070074
Received: 13 May 2021 / Revised: 11 June 2021 / Accepted: 21 June 2021 / Published: 22 June 2021
The use of fluorescence spectroscopy for plaque detection is a fast and effective way to monitor oral health. At present, there is no uniform specification for the design of the excitation light source of related products for generating fluorescence. To carry out experiments on dental plaque, the fluorescence spectra of three different bacterial species (Porphyromonas gingivalis, Aggregatibacter actinomycetemcomitans, and Streptococcus mutans) were measured by hyperspectral imaging microscopy (HIM). Three critical issues were found in the experiments. One issue was the unwanted spectrum generated from a mercury line source; two four-order low-pass filters were evaluated for eliminating the unwanted spectrum and meet the experimental requirements. The second issue was the red fluorescence generated from the microscope slide made of borosilicate glass; this could affect the observation of the red fluorescence from the bacteria; quartz microscope slides were found to reduce the fluorescence intensity by about 2 dB compared with the borosilicate slide. The third issue of photobleaching in the fluorescence of the Porphyromonas gingivalis was studied. This study proposes a method of classifying three bacteria based on the spectral intensity ratios (510/635 and 500/635 nm) under the 405 nm excitation light was proposed in this study. The sensitivity and specificity of the classification were approximately 99% and 99%, respectively. View Full-Text
Keywords: plaque detection; dental plaque; autofluorescence; Porphyromonas gingivalis; Aggregatibacter actinomycetemcomitans; Streptococcus mutans plaque detection; dental plaque; autofluorescence; Porphyromonas gingivalis; Aggregatibacter actinomycetemcomitans; Streptococcus mutans
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MDPI and ACS Style

Yan, Y.-J.; Wang, B.-W.; Yang, C.-M.; Wu, C.-Y.; Ou-Yang, M. Autofluorescence Detection Method for Dental Plaque Bacteria Detection and Classification: Example of Porphyromonas gingivalis, Aggregatibacter actinomycetemcomitans, and Streptococcus mutans. Dent. J. 2021, 9, 74. https://doi.org/10.3390/dj9070074

AMA Style

Yan Y-J, Wang B-W, Yang C-M, Wu C-Y, Ou-Yang M. Autofluorescence Detection Method for Dental Plaque Bacteria Detection and Classification: Example of Porphyromonas gingivalis, Aggregatibacter actinomycetemcomitans, and Streptococcus mutans. Dentistry Journal. 2021; 9(7):74. https://doi.org/10.3390/dj9070074

Chicago/Turabian Style

Yan, Yung-Jhe, Bo-Wen Wang, Chih-Man Yang, Ching-Yi Wu, and Mang Ou-Yang. 2021. "Autofluorescence Detection Method for Dental Plaque Bacteria Detection and Classification: Example of Porphyromonas gingivalis, Aggregatibacter actinomycetemcomitans, and Streptococcus mutans" Dentistry Journal 9, no. 7: 74. https://doi.org/10.3390/dj9070074

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