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Review

Flow Cytometry as a Valuable Tool to Study Cyanobacteria: A Mini-Review

by
Barbara Poniedziałek
1,*,
Halina I. Falfushynska
2 and
Piotr Rzymski
1
1
Department of Environmental Medicine, Poznan University of Medical Sciences, Rokietnicka 8, 60-806 Poznań, Poland
2
Research Laboratory of Comparative Biochemistry and Molecular Biology, Department of Health Care, Physical Rehabilitation and Vital Activity Safety, Ternopil V. Hnatiuk National Pedagogical University, M. Kryvonosa 2, 46027 Ternopil, Ukraine
*
Author to whom correspondence should be addressed.
Limnol. Rev. 2017, 17(2), 89-95; https://doi.org/10.1515/limre-2017-0009 (registering DOI)
Published: 19 December 2017

Abstract

Flow cytometry (FCM) is routinely used in medical and veterinary diagnostics although it is also widely applied in environmental studies, including phytoplankton investigations. Cyanobacteria are wide-spread photosynthetic microorganisms that attract attention due to their ecology and potential toxicity. Therefore, novel research tools are being applied in their investigation. This paper characterizes FCM as a technique that enables photopigments (chlorophylls and phycocyanin) expressed by cyanobacteria to be excited and their emission to be subsequently detected. This feature not only allows cells to be counted in a rapid manner but also enables a wide range of potential applications in ecological and biochemical studies. The main advantages of FCM, such as rapid, automatic and precise measurements requiring small sample volumes, are also discussed in this paper along with challenges including analyses of filamentous cyanobacteria and signal overlapping. It is expected that FCM will continue to be used in some fields of cyanobacterial studies.
Keywords: flow cytometry; cyanobacteria; fluorescence; chlorophyll; phycocyanin flow cytometry; cyanobacteria; fluorescence; chlorophyll; phycocyanin

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MDPI and ACS Style

Poniedziałek, B.; Falfushynska, H.I.; Rzymski, P. Flow Cytometry as a Valuable Tool to Study Cyanobacteria: A Mini-Review. Limnol. Rev. 2017, 17, 89-95. https://doi.org/10.1515/limre-2017-0009

AMA Style

Poniedziałek B, Falfushynska HI, Rzymski P. Flow Cytometry as a Valuable Tool to Study Cyanobacteria: A Mini-Review. Limnological Review. 2017; 17(2):89-95. https://doi.org/10.1515/limre-2017-0009

Chicago/Turabian Style

Poniedziałek, Barbara, Halina I. Falfushynska, and Piotr Rzymski. 2017. "Flow Cytometry as a Valuable Tool to Study Cyanobacteria: A Mini-Review" Limnological Review 17, no. 2: 89-95. https://doi.org/10.1515/limre-2017-0009

APA Style

Poniedziałek, B., Falfushynska, H. I., & Rzymski, P. (2017). Flow Cytometry as a Valuable Tool to Study Cyanobacteria: A Mini-Review. Limnological Review, 17(2), 89-95. https://doi.org/10.1515/limre-2017-0009

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