Next Article in Journal
HPLC-UV Polyphenolic Profiles in the Classification of Olive Oils and Other Vegetable Oils via Principal Component Analysis
Next Article in Special Issue
Separation and Purification with a Liquid Stationary Phase
Previous Article in Journal / Special Issue
Comparison in Partition Efficiency of Protein Separation between Four Different Tubing Modifications in Spiral High-Speed Countercurrent Chromatography
Article Menu

Export Article

Open AccessFeature PaperArticle
Separations 2016, 3(4), 32;

Dispersed Mobile-Phase Countercurrent Chromatography

Division of Life Science and Applied Genomics Center, Hong Kong University of Science and Technology, Clear Water Bay, Hong Kong, China
Author to whom correspondence should be addressed.
Academic Editor: Alain Berthod
Received: 27 July 2016 / Revised: 20 September 2016 / Accepted: 11 October 2016 / Published: 1 November 2016
(This article belongs to the Special Issue Countercurrent Chromatography)
Full-Text   |   PDF [1976 KB, uploaded 1 November 2016]   |  


Countercurrent distribution based on liquid–liquid partition is a powerful separation method with minimal incurrence of loss of solutes, but its industrial application has been limited by cumbersome shifting of immiscible solvents. Although centrifugation has been employed to facilitate equilibration between phases, process scaling-up remains difficult. In this study, a dispersed mobile-phase countercurrent chromatography (DMCC) method has been developed to adapt the countercurrent distribution principle to a continuous column chromatography format. Continuous solute exchange between two immiscible phases within a series of separation columns is achieved by mechanical dispersion of an influx of mobile phase into an upward stream of small droplets travelling through the columns filled with stationary phase. The diameter, length, and number of columns, and the number of stationary phases employed in the different columns can be varied to match the requisite scale and resolution of operation. Illustrations of DMCC were provided by examples of solute separations where the fractionated solutes could be collected either from the eluate of the series of columns, or from drainage of the stationary phases in the individual columns at the end of a chromatographic run. View Full-Text
Keywords: liquid–liquid partition; countercurrent chromatography; DMCC liquid–liquid partition; countercurrent chromatography; DMCC

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

Share & Cite This Article

MDPI and ACS Style

Ho, T.Y.-C.; Xue, H. Dispersed Mobile-Phase Countercurrent Chromatography. Separations 2016, 3, 32.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Metrics

Article Access Statistics



[Return to top]
Separations EISSN 2297-8739 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top