Rapid Purification and Quantification of Intestinal and Fecal Short-Chain Fatty Acids by Solid-Phase Extraction Using Bond Elut Plexa
Abstract
:1. Introduction
2. Materials and Methods
2.1. Samples and Reagents
2.2. SCFAs’ Standard Preparation and GC Analysis
2.3. Optimization of Extraction Method of SCFA from Samples
2.4. Recovery Assay and Reproducibility
2.5. SCFA Extraction from Fecal and Intestinal Content Samples
2.6. Statistical Analysis
3. Results and Discussion
3.1. Chromatography and Standard Curves
3.2. Optimization of Extraction Conditions
3.3. Recovery and Reproducibility
3.4. Quantitative Analysis of SCFA in Fecal and Intestinal Content Samples
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Conflicts of Interest
References
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SCFA Standards | L*1 (μM) | L2 (μM) | L3 (μM) | L4 (μM) | L5 (μM) | L6 (μM) |
---|---|---|---|---|---|---|
H*1 (μM) | H2 (μM) | H3 (μM) | H4 (μM) | H5 (μM) | ||
Acetic acid | 1074.07 | 537.03 | 107.41 | 53.70 | 10.74 | 1.07 |
Propionic acid | 803.19 | 401.59 | 80.32 | 40.16 | 8.03 | 0.80 |
Isobutyric acid | 631.07 | 315.53 | 63.11 | 31.55 | 6.31 | 0.63 |
Butyric acid | 649.23 | 324.61 | 64.92 | 32.46 | 6.49 | 0.65 |
Isovaleric acid | 535.58 | 267.79 | 53.56 | 26.78 | 5.36 | 0.54 |
Valeric acid | 543.41 | 271.71 | 54.34 | 27.17 | 5.43 | 0.54 |
Hexanoic acid | 458.86 | 229.43 | 45.89 | 22.94 | 4.59 | 0.46 |
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Zheng, X.; Chen, T.; Li, W.; Wang, K.; Xue, X.; Naumovski, N.; Peng, W. Rapid Purification and Quantification of Intestinal and Fecal Short-Chain Fatty Acids by Solid-Phase Extraction Using Bond Elut Plexa. Separations 2024, 11, 270. https://doi.org/10.3390/separations11090270
Zheng X, Chen T, Li W, Wang K, Xue X, Naumovski N, Peng W. Rapid Purification and Quantification of Intestinal and Fecal Short-Chain Fatty Acids by Solid-Phase Extraction Using Bond Elut Plexa. Separations. 2024; 11(9):270. https://doi.org/10.3390/separations11090270
Chicago/Turabian StyleZheng, Xing, Tao Chen, Wanli Li, Kai Wang, Xiaofeng Xue, Nenad Naumovski, and Wenjun Peng. 2024. "Rapid Purification and Quantification of Intestinal and Fecal Short-Chain Fatty Acids by Solid-Phase Extraction Using Bond Elut Plexa" Separations 11, no. 9: 270. https://doi.org/10.3390/separations11090270
APA StyleZheng, X., Chen, T., Li, W., Wang, K., Xue, X., Naumovski, N., & Peng, W. (2024). Rapid Purification and Quantification of Intestinal and Fecal Short-Chain Fatty Acids by Solid-Phase Extraction Using Bond Elut Plexa. Separations, 11(9), 270. https://doi.org/10.3390/separations11090270