Lipase Production by Solid-State Cultivation of Thermomyces Lanuginosus on By-Products from Cold-Pressing Oil Production
Abstract
:1. Introduction
2. Materials and Methods
2.1. Substrates
2.2. Microorganism and Commercial Enzyme
2.3. Enzyme Production
2.3.1. Solid-State Fermentation
2.3.2. Enzyme Purification
2.4. Analytical Methods
2.4.1. Measurements of Lipase Activity
2.4.2. Measurement of Fatty Acid Methyl Esters (FAME)
2.4.3. Enzyme Characterization
2.4.4. Biodiesel Production
2.4.5. Data Processing
3. Results
3.1. Lipase Production During Solid-State Cultivation of T. Lanuginosus on Oil Cakes
3.2. Enzyme Purification
3.3. Enzyme Characterization
3.4. Biodiesel Production
4. Conclusions and Future Work
Author Contributions
Funding
Conflicts of Interest
References
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Tišma, M.; Tadić, T.; Budžaki, S.; Ostojčić, M.; Šalić, A.; Zelić, B.; Tran, N.N.; Ngothai, Y.; Hessel, V. Lipase Production by Solid-State Cultivation of Thermomyces Lanuginosus on By-Products from Cold-Pressing Oil Production. Processes 2019, 7, 465. https://doi.org/10.3390/pr7070465
Tišma M, Tadić T, Budžaki S, Ostojčić M, Šalić A, Zelić B, Tran NN, Ngothai Y, Hessel V. Lipase Production by Solid-State Cultivation of Thermomyces Lanuginosus on By-Products from Cold-Pressing Oil Production. Processes. 2019; 7(7):465. https://doi.org/10.3390/pr7070465
Chicago/Turabian StyleTišma, Marina, Toma Tadić, Sandra Budžaki, Marta Ostojčić, Anita Šalić, Bruno Zelić, Nghiep Nam Tran, Yung Ngothai, and Volker Hessel. 2019. "Lipase Production by Solid-State Cultivation of Thermomyces Lanuginosus on By-Products from Cold-Pressing Oil Production" Processes 7, no. 7: 465. https://doi.org/10.3390/pr7070465