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Open AccessFeature PaperArticle

Lipase Production by Solid-State Cultivation of Thermomyces Lanuginosus on By-Products from Cold-Pressing Oil Production

1
Faculty of Food Technology Osijek, Josip Juraj Strossmayer University of Osijek, Osijek HR-31000, Croatia
2
Faculty of Chemical Engineering and Technology, University of Zagreb, Zagreb HR-10000, Croatia
3
School of Chemical Engineering, The University of Adelaide, North Terrace Campus, Adelaide 5005, Australia
4
School of Chemical Engineering, Can Tho University, Campus 2, Can Tho 900000, Vietnam
*
Author to whom correspondence should be addressed.
Processes 2019, 7(7), 465; https://doi.org/10.3390/pr7070465
Received: 13 June 2019 / Revised: 13 July 2019 / Accepted: 17 July 2019 / Published: 19 July 2019
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PDF [1290 KB, uploaded 19 July 2019]
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Abstract

This study shows that by-products obtained after cold-pressing oil production (flex oil cake, hemp oil cake, hull-less pumpkin oil cake) could be used as substrates for the sustainable and cost-effective production of lipase when cultivating Thermomyces lanuginosus under solid-state conditions (T = 45 °C, t = 9 days). Lipase showed optimum activity at T = 40 °C. The produced lipase extract was purified 17.03-folds with a recovery of 1% after gel chromatography. Three different batch experiments were performed in order to test the possibility of using the lipase in biodiesel production. Experiments were performed with a commercial, unpurified enzyme, and partially purified lipase with sunflower oil and methanol as substrates in a batch reactor at 40 °C. During the experiments, the operational stability of the enzyme was studied. The obtained results clearly showed that produced crude and purified lipase can be used for biodiesel production, but the process needs some additional optimization. As for operation stability, it was noticed that the commercial enzyme was deactivated after 30 h, while produced crude enzyme remained 8.25% of its activity after 368 h. View Full-Text
Keywords: flax oil cake; hemp oil cake; hull-less pumpkin oil cake; solid-state fermentation; lipase; Thermomyces lanuginosus flax oil cake; hemp oil cake; hull-less pumpkin oil cake; solid-state fermentation; lipase; Thermomyces lanuginosus
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
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Tišma, M.; Tadić, T.; Budžaki, S.; Ostojčić, M.; Šalić, A.; Zelić, B.; Tran, N.N.; Ngothai, Y.; Hessel, V. Lipase Production by Solid-State Cultivation of Thermomyces Lanuginosus on By-Products from Cold-Pressing Oil Production. Processes 2019, 7, 465.

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