Optimization of Baicalin, Wogonoside, and Chlorogenic Acid Water Extraction Process from the Roots of Scutellariae Radix and Lonicerae japonicae Flos Using Response Surface Methodology (RSM)

Li, Jichang; Wang, Rui; Sheng, Zunlai; Wu, Zhiyong; Chen, Chunli; Ishfaq, Muhammad

doi:10.3390/pr7110854

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Optimization of Baicalin, Wogonoside, and Chlorogenic Acid Water Extraction Process from the Roots of Scutellariae Radix and Lonicerae japonicae Flos Using Response Surface Methodology (RSM)

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College of Veterinary Medicine, Northeast Agricultural University, 600 Changjiang Road, Xiangfang District, Harbin 150030, China

Heilongjiang Key Laboratory for Animal Disease Control and Pharmaceutical Development, 600 Changjiang Road, Xiangfang District, Harbin 150030, China

Authors to whom correspondence should be addressed.

Processes 2019, 7(11), 854; https://doi.org/10.3390/pr7110854

Received: 18 October 2019 / Revised: 6 November 2019 / Accepted: 11 November 2019 / Published: 14 November 2019

(This article belongs to the Special Issue Advances in Green Chemistry Analytical Techniques)

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Abstract

In this study, a simultaneous water extraction process for baicalin, wogonoside, and chlorogenic acid has been optimized. The effect of extraction temperature, extraction time, and liquid–solid ratio was scrutinized by single factor experiments and further analyzed by Box–Behnken design (BBD) approach using response surface methodology (RSM). The extraction yield of investigated compounds was determined by high performance liquid chromatography (HPLC). Single-factor experiments and response surface analysis results revealed that the optimized conditions are: Liquid to solid ratio 25:1 (mL/g), extraction temperature 93 °C, extraction time 2.4 h, and the extraction cycle two. Importantly, it has been noted that under the above conditions, concentrations of baicalin, wogonoside, and chlorogenic were 0.078, 0.031, and 0.013 mg/mL, respectively, and the overall desirability (OD) value was 0.76 which was higher than the non-optimized conditions and the deviation from the predicted OD value was only 2.44%. Conclusively, it has been suggested that the model was stable and feasible, and fit for extraction of baicalin, wogonoside, and chlorogenic acid from Scutellariae Radix and Lonicerae (L.) japonicae Flos. View Full-Text

Keywords: baicalin; wogonoside; chlorogenic acid; extraction process; HPLC

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MDPI and ACS Style

Li, J.; Wang, R.; Sheng, Z.; Wu, Z.; Chen, C.; Ishfaq, M. Optimization of Baicalin, Wogonoside, and Chlorogenic Acid Water Extraction Process from the Roots of Scutellariae Radix and Lonicerae japonicae Flos Using Response Surface Methodology (RSM). Processes 2019, 7, 854. https://doi.org/10.3390/pr7110854

AMA Style

Li J, Wang R, Sheng Z, Wu Z, Chen C, Ishfaq M. Optimization of Baicalin, Wogonoside, and Chlorogenic Acid Water Extraction Process from the Roots of Scutellariae Radix and Lonicerae japonicae Flos Using Response Surface Methodology (RSM). Processes. 2019; 7(11):854. https://doi.org/10.3390/pr7110854

Chicago/Turabian Style

Li, Jichang, Rui Wang, Zunlai Sheng, Zhiyong Wu, Chunli Chen, and Muhammad Ishfaq. 2019. "Optimization of Baicalin, Wogonoside, and Chlorogenic Acid Water Extraction Process from the Roots of Scutellariae Radix and Lonicerae japonicae Flos Using Response Surface Methodology (RSM)" Processes 7, no. 11: 854. https://doi.org/10.3390/pr7110854

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Optimization of Baicalin, Wogonoside, and Chlorogenic Acid Water Extraction Process from the Roots of Scutellariae Radix and Lonicerae japonicae Flos Using Response Surface Methodology (RSM)

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