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Processes 2018, 6(4), 30; https://doi.org/10.3390/pr6040030

Genome-Scale In Silico Analysis for Enhanced Production of Succinic Acid in Zymomonas mobilis

1
Department of Chemical and Biomolecular Engineering, National University of Singapore, 4 Engineering Drive 4, Singapore 117585, Singapore
2
NUS Synthetic Biology for Clinical and Technological Innovation (SynCTI), Life Sciences Institute, National University of Singapore, 28 Medical Drive, Singapore 117456, Singapore
3
Bioprocessing Technology Institute, Agency for Science, Technology and Research (A*STAR), 20 Biopolis Way, #06-01, Centros, Singapore 138668, Singapore
4
School of Chemical Engineering, Sungkyunkwan University, 2066 Seobu-ro, Jangan-gu, Suwon, Gyeonggi-do 16419, Korea
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Received: 18 January 2018 / Revised: 12 March 2018 / Accepted: 26 March 2018 / Published: 1 April 2018
(This article belongs to the Special Issue Biological Networks)
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Abstract

Presented herein is a model-driven strategy for characterizing the production capability of expression host and subsequently identifying targets for strain improvement by resorting to network structural comparison with reference strain and in silico analysis of genome-scale metabolic model. The applicability of the strategy was demonstrated by exploring the capability of Zymomonas mobilis, as a succinic acid producer. Initially, the central metabolism of Z. mobilis was compared with reference producer, Mannheimia succiniciproducens, in order to identify gene deletion targets. It was followed by combinatorial gene deletion analysis. Remarkably, resultant in silico strains suggested that knocking out pdc, ldh, and pfl genes encoding pyruvate-consuming reactions as well as the cl gene leads to fifteen-fold increase in succinic acid molar yield. The current exploratory work could be a promising support to wet experiments by providing guidance for metabolic engineering strategies and lowering the number of trials and errors. View Full-Text
Keywords: Zymomonas mobilis; succinic acid; gene deletion; genome-scale metabolic model; systems biology Zymomonas mobilis; succinic acid; gene deletion; genome-scale metabolic model; systems biology
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Widiastuti, H.; Lee, N.-R.; Karimi, I.A.; Lee, D.-Y. Genome-Scale In Silico Analysis for Enhanced Production of Succinic Acid in Zymomonas mobilis. Processes 2018, 6, 30.

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