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Processes 2018, 6(3), 21; https://doi.org/10.3390/pr6030021

Mathematical Modeling and Parameter Estimation of Intracellular Signaling Pathway: Application to LPS-induced NFκB Activation and TNFα Production in Macrophages

1
Artie McFerrin Department of Chemical Engineering, Texas A&M University, College Station, TX 77843, USA
2
Texas A&M Energy Institute, Texas A&M University, College Station, TX 77843, USA
3
Department of Biomedical Engineering, Texas A&M University, College Station, TX 77843, USA
*
Author to whom correspondence should be addressed.
Received: 28 January 2018 / Revised: 10 February 2018 / Accepted: 21 February 2018 / Published: 25 February 2018
(This article belongs to the Special Issue Biological Networks)
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Abstract

Due to the intrinsic stochasticity, the signaling dynamics in a clonal population of cells exhibit cell-to-cell variability at the single-cell level, which is distinct from the population-average dynamics. Frequently, flow cytometry is widely used to acquire the single-cell level measurements by blocking cytokine secretion with reagents such as Golgiplug. However, Golgiplug can alter the signaling dynamics, causing measurements to be misleading. Hence, we developed a mathematical model to infer the average single-cell dynamics based on the flow cytometry measurements in the presence of Golgiplug with lipopolysaccharide (LPS)-induced NF κ B signaling as an example. First, a mathematical model was developed based on the prior knowledge. Then, average single-cell dynamics of two key molecules (TNF α and I κ B α ) in the NF κ B signaling pathway were measured through flow cytometry in the presence of Golgiplug to validate the model and maximize its prediction accuracy. Specifically, a parameter selection and estimation scheme selected key model parameters and estimated their values. Unsatisfactory results from the parameter estimation guided subsequent experiments and appropriate model improvements, and the refined model was calibrated again through the parameter estimation. The inferred model was able to make predictions that were consistent with the experimental measurements, which will be used to construct a semi-stochastic model in the future. View Full-Text
Keywords: systems biology; parameter estimation; NFκB signaling pathway; lipopolysaccharide; flow cytometry; sensitivity analysis systems biology; parameter estimation; NFκB signaling pathway; lipopolysaccharide; flow cytometry; sensitivity analysis
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Lee, D.; Ding, Y.; Jayaraman, A.; Kwon, J.S. Mathematical Modeling and Parameter Estimation of Intracellular Signaling Pathway: Application to LPS-induced NFκB Activation and TNFα Production in Macrophages. Processes 2018, 6, 21.

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