Ultrasound-Mediated DNA Transformation of Bacteria
Abstract
:1. Introduction
2. Materials and Methods
2.1. Strain and Plasmids
2.2. Cell Culture and Preparation of Receptive Cells
2.3. Ultrasonic Conversion Process
2.4. Transformant Identification
2.5. Electron Microscope Analysis
2.6. Statistical Analysis
3. Results and Discussion
3.1. Ultrasound-Mediated Plasmid Transformation of E. coli
3.2. Electron Microscopy
3.3. Ultrasound-Mediated Plasmid Transformation of Saccharomyces cerevisiae
4. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Name | Description | Reference/Sources |
---|---|---|
STRAIN | ||
E. coli DH5α | supE44 Δ(lacZYA-argF) U169 (Φ80lacZ ΔM15) hsdR17 recA endA1 | Invitrogen |
S. cerevisiae S288C | MATα SUC2 gal2 mal2 mel flo1 flo8-1 hap1 ho bio1 bio6 | ATCC 204508 |
S. cerevisiae NGT-F1 | derived from S. cerevisiae S288C, and tolerance to 381 g/L source | [19] |
PLASMID | ||
pZEABP | constitute expression vector, pBR322 ori, P37 promoter, Ampr, BglBrick, ePathBrick containing four isocaudomers (AvrII, NheI, SpeI, and XbaI) | [20] |
pUG6 | Shuttle plasmid, Ampr in E. coli, G418r in S. cerevisiae | Novagen VT1696 |
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Wang, B.-P.; Yuan, Y.-M.; Yang, S.; Xu, Y.; Liao, C.-Y.; Niu, F.-X. Ultrasound-Mediated DNA Transformation of Bacteria. Processes 2023, 11, 2163. https://doi.org/10.3390/pr11072163
Wang B-P, Yuan Y-M, Yang S, Xu Y, Liao C-Y, Niu F-X. Ultrasound-Mediated DNA Transformation of Bacteria. Processes. 2023; 11(7):2163. https://doi.org/10.3390/pr11072163
Chicago/Turabian StyleWang, Bei-Ping, Yue-Mei Yuan, Sheng Yang, Yun Xu, Chun-Yan Liao, and Fu-Xing Niu. 2023. "Ultrasound-Mediated DNA Transformation of Bacteria" Processes 11, no. 7: 2163. https://doi.org/10.3390/pr11072163