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In Vitro Assessment of Migratory Behavior of Two Cell Populations in a Simple Multichannel Microdevice
Stem Cell Therapies Laboratory, Queensland University of Technology, Translational Research Institute, Brisbane 4102, Australia
Department of Biotechnology, College of Science, University of Tehran, Tehran, Iran
Department of Mechanical Engineering, Esfahan University of Technology, Esfahan, Iran
Australian Prostate Cancer Research Centre, Translational Research Institute, Brisbane 4102, Australia
Mater Medical Research Institute, Translational Research Institute, Brisbane 4102, Australia
* Author to whom correspondence should be addressed.
Received: 22 July 2013; in revised form: 17 October 2013 / Accepted: 20 November 2013 / Published: 18 December 2013
Abstract: Recent literature suggests that mesenchymal stem/stromal cells (MSC) could be used as Trojan Horses to deliver “death-signals” to cancer cells. Herein, we describe the development of a novel multichannel cell migration device, and use it to investigate the relative migration rates of bone marrow-derived MSC and breast cancer cells (MCF-7) towards each other. Confluent monolayers of MSC and MCF-7 were established in adjacent chambers separated by an array of 14 microchannels. Initially, culture chambers were isolated by air bubbles (air-valves) contained within each microchannel, and then bubbles were displaced to initiate the assay. The MCF-7 cells migrated preferentially towards MSC, whilst the MSC did not migrate preferentially towards the MCF-7 cells. Our results corroborate previous literature that suggests MSC migration towards cancer cells in vivo is in response to the associated inflammation rather than directly to signals secreted by the cancer cells themselves.
Keywords: cancer cells; MSC migration; multichannel microdevice; paracrine signaling
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MDPI and ACS Style
Kabiri, M.; Lott, W.B.; Kabiri, E.; Russell, P.J.; Doran, M.R. In Vitro Assessment of Migratory Behavior of Two Cell Populations in a Simple Multichannel Microdevice. Processes 2013, 1, 349-359.
Kabiri M, Lott WB, Kabiri E, Russell PJ, Doran MR. In Vitro Assessment of Migratory Behavior of Two Cell Populations in a Simple Multichannel Microdevice. Processes. 2013; 1(3):349-359.
Kabiri, Mahboubeh; Lott, William B.; Kabiri, Ehsan; Russell, Pamela J.; Doran, Michael R. 2013. "In Vitro Assessment of Migratory Behavior of Two Cell Populations in a Simple Multichannel Microdevice." Processes 1, no. 3: 349-359.