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Please note that, as of 1 January 2016, Chromatography has been renamed to Separations and is now published here.
Open AccessArticle

Adaptation of a High-Pressure Liquid Chromatography System for the Measurement of Viscosity

Merck Research Laboratories, Merck & Co., Inc., West Point, PA 19486, USA
Author to whom correspondence should be addressed.
Chromatography 2014, 1(2), 55-64;
Received: 27 January 2014 / Revised: 10 March 2014 / Accepted: 17 March 2014 / Published: 26 March 2014
The state-of-the-art instruments for the determination of viscosity of liquids typically require a significant amount of sample, and have relatively low throughput due to manual and sequential measurements. In this study, it was demonstrated that the pressure generated by the flow of viscous fluids through a capillary could be precisely measured employing high-pressure liquid chromatography systems (HPLC) using glycerol solutions of moderate viscosity as a mobile phase, and correlated to the dynamic (absolute) viscosity. The parameters allowing calculation of the viscosity of glycerol calibration standards as a function of temperature were established. The measurements were made with volumes as small as 10 μL, and the use of an autosampler permitted unattended analysis of a large number samples. The method appears to be particularly well suited for the development of viscous formulations of therapeutic, protein-based macromolecules, where the amount sample is typically limited and relatively wide ranges of conditions are considered in the optimization process. The utility of the methods was illustrated by application to the development of concentrated inactivated virus vaccines. View Full-Text
Keywords: viscosity; high-pressure liquid chromatography; protein formulation viscosity; high-pressure liquid chromatography; protein formulation
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MDPI and ACS Style

Gregory, S.; Mach, H. Adaptation of a High-Pressure Liquid Chromatography System for the Measurement of Viscosity. Chromatography 2014, 1, 55-64.

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