Quantitative Approaches to Study Retinal Neurogenesis
Abstract
:1. Introduction
2. Methods
2.1. Animals
2.2. Sample Preparation
2.3. Immunostaining and Mounting
2.4. Image Acquisition
2.5. Object Density Analysis
3. From Qualitative to Quantitative
4. 2D Quantitative Data
5. Automated Image Analysis
6. The Developing Vertebrate Retina in Three-Dimensions
7. The Developing Vertebrate Retina in Four-Dimensions
8. Discussion
9. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
Sample Availability
Abbreviations
RPC | Retinal progenitor cell |
HPF | Hours post fertilization |
mInsc | Mammalian homolog of Inscuteable |
LSFM | Light sheet fluorescence microscopy |
SPIM | Selective plane illumination microscopy |
iPS | Induced pluripotent stem |
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Pérez-Dones, D.; Ledesma-Terrón, M.; Míguez, D.G. Quantitative Approaches to Study Retinal Neurogenesis. Biomedicines 2021, 9, 1222. https://doi.org/10.3390/biomedicines9091222
Pérez-Dones D, Ledesma-Terrón M, Míguez DG. Quantitative Approaches to Study Retinal Neurogenesis. Biomedicines. 2021; 9(9):1222. https://doi.org/10.3390/biomedicines9091222
Chicago/Turabian StylePérez-Dones, Diego, Mario Ledesma-Terrón, and David G. Míguez. 2021. "Quantitative Approaches to Study Retinal Neurogenesis" Biomedicines 9, no. 9: 1222. https://doi.org/10.3390/biomedicines9091222