A 3D In Vitro Model for Burn Wounds: Monitoring of Regeneration on the Epidermal Level
Abstract
:1. Introduction
2. Materials and Methods
2.1. Isolation and Culture of Primary Skin Cells
2.2. Generation of Epidermis Models
2.3. Burning of Epidermis Models
2.4. CEDEX Glucose Metabolism
2.5. Barrier Function Impedance
2.6. Viability Measurement (MTT Assay)
2.7. Measuring of the Burned Surface Area
2.8. Histological Staining and Immunofluorescence
2.9. Quantitative Analysis of Histological Sections Using a Scoring System
2.10. Cytometric Bead Assay
2.11. Statistical Analysis
3. Results
3.1. Burn Wounds Can Be Generated with a Heated Metal Rod and Regenerate over 14 Days
3.2. Wound Healing Can Be Monitored Using Histological and Immunohistological Analysis
3.3. Barrier Integrity, LDH Release and Metabolic Changes Can Be Measured in Wound Models
3.4. Burn Wounds Cause Inflammatory Activity in Reconstructed Human Epidermis
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Schneider, V.; Kruse, D.; de Mattos, I.B.; Zöphel, S.; Tiltmann, K.-K.; Reigl, A.; Khan, S.; Funk, M.; Bodenschatz, K.; Groeber-Becker, F. A 3D In Vitro Model for Burn Wounds: Monitoring of Regeneration on the Epidermal Level. Biomedicines 2021, 9, 1153. https://doi.org/10.3390/biomedicines9091153
Schneider V, Kruse D, de Mattos IB, Zöphel S, Tiltmann K-K, Reigl A, Khan S, Funk M, Bodenschatz K, Groeber-Becker F. A 3D In Vitro Model for Burn Wounds: Monitoring of Regeneration on the Epidermal Level. Biomedicines. 2021; 9(9):1153. https://doi.org/10.3390/biomedicines9091153
Chicago/Turabian StyleSchneider, Verena, Daniel Kruse, Ives Bernardelli de Mattos, Saskia Zöphel, Kendra-Kathrin Tiltmann, Amelie Reigl, Sarah Khan, Martin Funk, Karl Bodenschatz, and Florian Groeber-Becker. 2021. "A 3D In Vitro Model for Burn Wounds: Monitoring of Regeneration on the Epidermal Level" Biomedicines 9, no. 9: 1153. https://doi.org/10.3390/biomedicines9091153