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Article

Differentiation of Adipose-Derived Stem Cells into Vascular Smooth Muscle Cells for Tissue Engineering Applications

1
Human Health Therapeutics, National Research Council of Canada, 1200 Montreal Road, Ottawa, ON K1A0R6, Canada
2
Medical Devices Research Centre, National Research Council of Canada, 435 Ellice Ave, Winnipeg, MB R3B 1Y6, Canada
*
Authors to whom correspondence should be addressed.
Academic Editor: María García-Díaz
Biomedicines 2021, 9(7), 797; https://doi.org/10.3390/biomedicines9070797
Received: 21 May 2021 / Revised: 15 June 2021 / Accepted: 25 June 2021 / Published: 9 July 2021
(This article belongs to the Special Issue Bioengineered In Vitro Models for Biomedical Applications)
Synthetic grafts have been developed for vascular bypass surgery, however, the risks of thrombosis and neointimal hyperplasia still limit their use. Tissue engineering with the use of adipose-derived stem cells (ASCs) has shown promise in addressing these limitations. Here we further characterized and optimized the ASC differentiation into smooth muscle cells (VSMCs) induced by TGF-β and BMP-4. TGF-β and BMP-4 induced a time-dependent expression of SMC markers in ASC. Shortening the differentiation period from 7 to 4 days did not impair the functional property of contraction in these cells. Stability of the process was demonstrated by switching cells to regular growth media for up to 14 days. The role of IGFBP7, a downstream effector of TGF-β, was also examined. Finally, topographic and surface patterning of a substrate is recognized as a powerful tool for regulating cell differentiation. Here we provide evidence that a non-woven PET structure does not affect the differentiation of ASC. Taken together, our results indicate that VSMCs differentiated from ASCs are a suitable candidate to populate a PET-based vascular scaffolds. By employing an autologous source of cells we provide a novel alternative to address major issues that reduces long-term patency of currently vascular grafts. View Full-Text
Keywords: adipose stem cells; tissue engineering; differentiation; vascular smooth muscle; contraction adipose stem cells; tissue engineering; differentiation; vascular smooth muscle; contraction
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MDPI and ACS Style

Yogi, A.; Rukhlova, M.; Charlebois, C.; Tian, G.; Stanimirovic, D.B.; Moreno, M.J. Differentiation of Adipose-Derived Stem Cells into Vascular Smooth Muscle Cells for Tissue Engineering Applications. Biomedicines 2021, 9, 797. https://doi.org/10.3390/biomedicines9070797

AMA Style

Yogi A, Rukhlova M, Charlebois C, Tian G, Stanimirovic DB, Moreno MJ. Differentiation of Adipose-Derived Stem Cells into Vascular Smooth Muscle Cells for Tissue Engineering Applications. Biomedicines. 2021; 9(7):797. https://doi.org/10.3390/biomedicines9070797

Chicago/Turabian Style

Yogi, Alvaro, Marina Rukhlova, Claudie Charlebois, Ganghong Tian, Danica B. Stanimirovic, and Maria J. Moreno. 2021. "Differentiation of Adipose-Derived Stem Cells into Vascular Smooth Muscle Cells for Tissue Engineering Applications" Biomedicines 9, no. 7: 797. https://doi.org/10.3390/biomedicines9070797

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