N-acetylcysteine Provides Cytoprotection in Murine Oligodendrocytes through Heme Oxygenase-1 Activity
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials
2.2. Cell Culture and Experimental Conditions
2.3. Cell Survival Assays
2.4. Evaluation of ROS Production
2.5. Determination of Intracellular GSH
2.6. Total Antioxidant Capacity Assay
2.7. Statistical Data Analysis
3. Results
3.1. NAC Decreases ROS Content in Oligodendrocytes
3.2. NAC Improves 158N Cell Survival in Oxidative Stress
3.3. GSH Depletion Partially Reduces the Cytoprotective Effect of NAC
3.4. HO-1 Activity Mediate the Protective Effect of NAC
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Zhou, J.; Terluk, M.R.; Basso, L.; Mishra, U.R.; Orchard, P.J.; Cloyd, J.C.; Schröder, H.; Kartha, R.V. N-acetylcysteine Provides Cytoprotection in Murine Oligodendrocytes through Heme Oxygenase-1 Activity. Biomedicines 2020, 8, 240. https://doi.org/10.3390/biomedicines8080240
Zhou J, Terluk MR, Basso L, Mishra UR, Orchard PJ, Cloyd JC, Schröder H, Kartha RV. N-acetylcysteine Provides Cytoprotection in Murine Oligodendrocytes through Heme Oxygenase-1 Activity. Biomedicines. 2020; 8(8):240. https://doi.org/10.3390/biomedicines8080240
Chicago/Turabian StyleZhou, Jie, Marcia R. Terluk, Lisa Basso, Usha R. Mishra, Paul J. Orchard, James C. Cloyd, Henning Schröder, and Reena V. Kartha. 2020. "N-acetylcysteine Provides Cytoprotection in Murine Oligodendrocytes through Heme Oxygenase-1 Activity" Biomedicines 8, no. 8: 240. https://doi.org/10.3390/biomedicines8080240