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Open AccessArticle

Atropine Differentially Modulates ECM Production by Ocular Fibroblasts, and Its Ocular Surface Toxicity Is Blunted by Colostrum

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Research Center, Sooft Italia SpA c/o Biologic Tower, University of Catania, 95123 Catania, Italy
2
Department of Biomedical and Biotechnological Sciences, Biologic Tower, University of Catania, 95123 Catania, Italy
*
Authors to whom correspondence should be addressed.
Biomedicines 2020, 8(4), 78; https://doi.org/10.3390/biomedicines8040078
Received: 26 February 2020 / Revised: 15 March 2020 / Accepted: 2 April 2020 / Published: 5 April 2020
(This article belongs to the Section Therapeutic Strategies in Different Diseases)
Background: The etiology and the mechanism behind atropine treatment of progressive myopia are still poorly understood. Our study addressed the role of scleral and choroidal fibroblasts in myopia development and atropine function. Methods: Fibroblasts treated in vitro with atropine or 7-methylxanthine were tested for ECM production by Western blotting. Corneal epithelial cells were treated with atropine in the presence or absence of colostrum or fucosyl-lactose, and cell survival was evaluated by the MTT metabolic test. Results: Atropine and 7-methyl-xanthine stimulated collagen I and fibronectin production in scleral fibroblasts, while they inhibited their production in choroidal fibroblasts. Four days of treatment with atropine of corneal epithelial cells significantly decreased cell viability, which could be prevented by the presence of colostrum or fucosyl-lactose. Conclusions: Our results show that atropine may function in different ways in different eye districts, strengthening the scleral ECM and increasing permeability in the choroid. The finding that colostrum or fucosyl-lactose attenuate the corneal epithelial toxicity after long-term atropine treatment suggests the possibility that both compounds can efficiently blunt its toxicity in children subjected to chronic atropine treatment. View Full-Text
Keywords: Atropine; 7-methyl-xanthine; myopia; collagen; sclera; choroid; colostrum; 2-fucosyl-lactose; fibroblasts Atropine; 7-methyl-xanthine; myopia; collagen; sclera; choroid; colostrum; 2-fucosyl-lactose; fibroblasts
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MDPI and ACS Style

Cristaldi, M.; Olivieri, M.; Pezzino, S.; Spampinato, G.; Lupo, G.; Anfuso, C.D.; Rusciano, D. Atropine Differentially Modulates ECM Production by Ocular Fibroblasts, and Its Ocular Surface Toxicity Is Blunted by Colostrum. Biomedicines 2020, 8, 78.

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