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Article

Verbascoside Protects Pancreatic β-Cells against ER-Stress

1
Department of Pharmacological and Biomolecular Sciences, Università degli Studi di Milano, 20134 Milan, Italy
2
Diabetology Unit, Niguarda Hospital, 20162 Milan, Italy
3
Department of Veterinary Medicine, Università degli Studi di Milano, 26900 Lodi, Italy
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Biomedicines 2020, 8(12), 582; https://doi.org/10.3390/biomedicines8120582
Received: 16 November 2020 / Revised: 4 December 2020 / Accepted: 5 December 2020 / Published: 8 December 2020
(This article belongs to the Special Issue Anti-inflammatory Activity of Plant Polyphenols 2.0)
Substantial epidemiological evidence indicates that a diet rich in polyphenols protects against developing type 2 diabetes. The phenylethanoid glycoside verbascoside/acteoside, a widespread polyphenolic plant compound, has several biological properties including strong antioxidant, anti-inflammatory and neuroprotective activities. The aim of this research was to test the possible effects of verbascoside on pancreatic β-cells, a target never tested before. Mouse and human β-cells were incubated with verbascoside (0.8–16 µM) for up to five days and a combination of biochemical and imaging techniques were used to assess the β-cell survival and function under normal or endoplasmic reticulum (ER)-stress inducing conditions. We found a dose-dependent protective effect of verbascoside against oxidative stress in clonal and human β-cells. Mechanistic studies revealed that the polyphenol protects β-cells against ER-stress mediated dysfunctions, modulating the activation of the protein kinase RNA-like endoplasmic reticulum kinase (PERK) branch of the unfolded protein response and promoting mitochondrial dynamics. As a result, increased viability, mitochondrial function and insulin content were detected in these cells. These studies provide the evidence that verbascoside boosts the ability of β-cells to cope with ER-stress, an important contributor of β-cell dysfunction and failure in diabetic conditions and support the therapeutic potential of verbascoside in diabetes. View Full-Text
Keywords: verbascoside; polyphenols; insulin-producing cells; diabetes; UPR; oxidative stress; ER-stress; PERK; anti-inflammatory; mitochondria verbascoside; polyphenols; insulin-producing cells; diabetes; UPR; oxidative stress; ER-stress; PERK; anti-inflammatory; mitochondria
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MDPI and ACS Style

Galli, A.; Marciani, P.; Marku, A.; Ghislanzoni, S.; Bertuzzi, F.; Rossi, R.; Di Giancamillo, A.; Castagna, M.; Perego, C. Verbascoside Protects Pancreatic β-Cells against ER-Stress. Biomedicines 2020, 8, 582. https://doi.org/10.3390/biomedicines8120582

AMA Style

Galli A, Marciani P, Marku A, Ghislanzoni S, Bertuzzi F, Rossi R, Di Giancamillo A, Castagna M, Perego C. Verbascoside Protects Pancreatic β-Cells against ER-Stress. Biomedicines. 2020; 8(12):582. https://doi.org/10.3390/biomedicines8120582

Chicago/Turabian Style

Galli, Alessandra, Paola Marciani, Algerta Marku, Silvia Ghislanzoni, Federico Bertuzzi, Raffaella Rossi, Alessia Di Giancamillo, Michela Castagna, and Carla Perego. 2020. "Verbascoside Protects Pancreatic β-Cells against ER-Stress" Biomedicines 8, no. 12: 582. https://doi.org/10.3390/biomedicines8120582

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