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Verbascoside Protects Pancreatic β-Cells against ER-Stress

Department of Pharmacological and Biomolecular Sciences, Università degli Studi di Milano, 20134 Milan, Italy
Diabetology Unit, Niguarda Hospital, 20162 Milan, Italy
Department of Veterinary Medicine, Università degli Studi di Milano, 26900 Lodi, Italy
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Biomedicines 2020, 8(12), 582;
Received: 16 November 2020 / Revised: 4 December 2020 / Accepted: 5 December 2020 / Published: 8 December 2020
(This article belongs to the Special Issue Anti-inflammatory Activity of Plant Polyphenols 2.0)
Substantial epidemiological evidence indicates that a diet rich in polyphenols protects against developing type 2 diabetes. The phenylethanoid glycoside verbascoside/acteoside, a widespread polyphenolic plant compound, has several biological properties including strong antioxidant, anti-inflammatory and neuroprotective activities. The aim of this research was to test the possible effects of verbascoside on pancreatic β-cells, a target never tested before. Mouse and human β-cells were incubated with verbascoside (0.8–16 µM) for up to five days and a combination of biochemical and imaging techniques were used to assess the β-cell survival and function under normal or endoplasmic reticulum (ER)-stress inducing conditions. We found a dose-dependent protective effect of verbascoside against oxidative stress in clonal and human β-cells. Mechanistic studies revealed that the polyphenol protects β-cells against ER-stress mediated dysfunctions, modulating the activation of the protein kinase RNA-like endoplasmic reticulum kinase (PERK) branch of the unfolded protein response and promoting mitochondrial dynamics. As a result, increased viability, mitochondrial function and insulin content were detected in these cells. These studies provide the evidence that verbascoside boosts the ability of β-cells to cope with ER-stress, an important contributor of β-cell dysfunction and failure in diabetic conditions and support the therapeutic potential of verbascoside in diabetes. View Full-Text
Keywords: verbascoside; polyphenols; insulin-producing cells; diabetes; UPR; oxidative stress; ER-stress; PERK; anti-inflammatory; mitochondria verbascoside; polyphenols; insulin-producing cells; diabetes; UPR; oxidative stress; ER-stress; PERK; anti-inflammatory; mitochondria
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MDPI and ACS Style

Galli, A.; Marciani, P.; Marku, A.; Ghislanzoni, S.; Bertuzzi, F.; Rossi, R.; Di Giancamillo, A.; Castagna, M.; Perego, C. Verbascoside Protects Pancreatic β-Cells against ER-Stress. Biomedicines 2020, 8, 582.

AMA Style

Galli A, Marciani P, Marku A, Ghislanzoni S, Bertuzzi F, Rossi R, Di Giancamillo A, Castagna M, Perego C. Verbascoside Protects Pancreatic β-Cells against ER-Stress. Biomedicines. 2020; 8(12):582.

Chicago/Turabian Style

Galli, Alessandra, Paola Marciani, Algerta Marku, Silvia Ghislanzoni, Federico Bertuzzi, Raffaella Rossi, Alessia Di Giancamillo, Michela Castagna, and Carla Perego. 2020. "Verbascoside Protects Pancreatic β-Cells against ER-Stress" Biomedicines 8, no. 12: 582.

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