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Open AccessArticle

Experimental Study on Blue Light Interaction with Human Keloid-Derived Fibroblasts

1
Istituto di Fisica Applicata “Nello Carrara”, Consiglio Nazionale delle Ricerche (CNR-IFAC), 50019 Florence, Italy
2
Department of Neuroscience, Psychology, Drug Research and Child Health, Section of Pharmacology and Toxicology, University of Florence, 50139 Florence, Italy
3
SCDU Chirurgia Plastica, ASO Città della Salute e della Scienza di Torino, 10133 Turin, Italy
4
EmoLED s.r.l., 50019, Florence, Italy
5
Department of Physics, University of Florence, 50019 Florence, Italy
6
European Laboratory for NonLinear Spectroscopy (LENS), 50019 Florence, Italy
7
Istituto Nazionale di Ottica, Consiglio Nazionale delle Ricerche (CNR-INO), 50125 Florence, Italy
*
Authors to whom correspondence should be addressed.
Biomedicines 2020, 8(12), 573; https://doi.org/10.3390/biomedicines8120573
Received: 9 November 2020 / Revised: 27 November 2020 / Accepted: 30 November 2020 / Published: 6 December 2020
(This article belongs to the Special Issue Cellular Mechanisms in Wound Healing)
Keloids are an exuberant response to wound healing, characterized by an exaggerated synthesis of collagen, probably due to the increase of fibroblasts activity and to the reduction of their apoptosis rate: currently no standard treatments or pharmacological therapies are able to prevent keloid recurrence. To reach this goal, in recent years some physical treatments have been proposed, and among them the PhotoBioModulation therapy (PBM). This work analyses the effects of a blue LED light irradiation (410–430 nm, 0.69 W/cm2 power density) on human fibroblasts, isolated from both keloids and perilesional tissues. Different light doses (3.43–6.87–13.7–20.6–30.9 and 41.2 J/cm2) were tested. Biochemical assays and specific staining were used to assess cell metabolism, proliferation and viability. Micro-Raman spectroscopy was used to explore direct effects of the blue LED light on the Cytochrome C (Cyt C) oxidase. We also investigated the effects of the irradiation on ionic membrane currents by patch-clamp recordings. Our results showed that the blue LED light can modulate cell metabolism and proliferation, with a dose-dependent behavior and that these effects persist at least till 48 h after treatment. Furthermore, we demonstrated that the highest fluence value can reduce cell viability 24 h after irradiation in keloid-derived fibroblasts, while the same effect is observed 48 h after treatment in perilesional fibroblasts. Electrophysiological recordings showed that the medium dose (20.6 J/cm2) of blue LED light induces an enhancement of voltage-dependent outward currents elicited by a depolarizing ramp protocol. Overall, these data demonstrate the potentials that PBM shows as an innovative and minimally-invasive approach in the management of hypertrophic scars and keloids, in association with current treatments. View Full-Text
Keywords: blue light; LED; photobiomodulation; skin fibrosis; keloid; fibroblast; Cytochrome C; Raman spectroscopy; patch-clamp blue light; LED; photobiomodulation; skin fibrosis; keloid; fibroblast; Cytochrome C; Raman spectroscopy; patch-clamp
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MDPI and ACS Style

Magni, G.; Banchelli, M.; Cherchi, F.; Coppi, E.; Fraccalvieri, M.; Rossi, M.; Tatini, F.; Pugliese, A.M.; Rossi Degl’Innocenti, D.; Alfieri, D.; Matteini, P.; Pini, R.; Pavone, F.S.; Rossi, F. Experimental Study on Blue Light Interaction with Human Keloid-Derived Fibroblasts. Biomedicines 2020, 8, 573.

AMA Style

Magni G, Banchelli M, Cherchi F, Coppi E, Fraccalvieri M, Rossi M, Tatini F, Pugliese AM, Rossi Degl’Innocenti D, Alfieri D, Matteini P, Pini R, Pavone FS, Rossi F. Experimental Study on Blue Light Interaction with Human Keloid-Derived Fibroblasts. Biomedicines. 2020; 8(12):573.

Chicago/Turabian Style

Magni, Giada; Banchelli, Martina; Cherchi, Federica; Coppi, Elisabetta; Fraccalvieri, Marco; Rossi, Michele; Tatini, Francesca; Pugliese, Anna M.; Rossi Degl’Innocenti, Duccio; Alfieri, Domenico; Matteini, Paolo; Pini, Roberto; Pavone, Francesco S.; Rossi, Francesca. 2020. "Experimental Study on Blue Light Interaction with Human Keloid-Derived Fibroblasts" Biomedicines 8, no. 12: 573.

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