Analgesic Tolerance Development during Repetitive Electric Stimulations Is Associated with Changes in the Expression of Activated Microglia in Rats with Osteoarthritis
Abstract
:1. Introduction
2. Materials and Methods
2.1. Experimental Animals
2.2. Induction of OA
2.3. TENS Application
2.4. Intrathecal Catheterization
2.5. Drug Delivery
2.6. Behavioral Analyses
2.7. Tissue Processing and Immunohistochemistry
2.8. Quantitative Image Analysis
2.9. Experimental Procedure
2.10. Statistics
3. Results
3.1. Changes of OA Pain by Repeated TENS in Rats with OA
3.2. Changes in Spinal Microglia due to Repeated TENS Applications in Rats with OA
3.3. Effects of Microglial Inhibition on OA Pain in TENS-Tolerant Rats
3.4. Effects of TENS with Microglia Inhibition on OA Pain in TENS-Tolerant Rats
3.5. Spinal Glial Changes Induced by TENS Treatment with 10-μg Minocycline in TENS-Tolerant Rats with OA
4. Discussion
5. Conclusions
Author Contributions
Funding
Conflicts of Interest
References
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Hahm, S.-C.; Lee, J.S.; Yoon, Y.W.; Kim, J. Analgesic Tolerance Development during Repetitive Electric Stimulations Is Associated with Changes in the Expression of Activated Microglia in Rats with Osteoarthritis. Biomedicines 2020, 8, 575. https://doi.org/10.3390/biomedicines8120575
Hahm S-C, Lee JS, Yoon YW, Kim J. Analgesic Tolerance Development during Repetitive Electric Stimulations Is Associated with Changes in the Expression of Activated Microglia in Rats with Osteoarthritis. Biomedicines. 2020; 8(12):575. https://doi.org/10.3390/biomedicines8120575
Chicago/Turabian StyleHahm, Suk-Chan, Jin Seung Lee, Young Wook Yoon, and Junesun Kim. 2020. "Analgesic Tolerance Development during Repetitive Electric Stimulations Is Associated with Changes in the Expression of Activated Microglia in Rats with Osteoarthritis" Biomedicines 8, no. 12: 575. https://doi.org/10.3390/biomedicines8120575