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Open AccessArticle

Continuous Electrical Stimulation Affects Initial Growth and Proliferation of Adipose-Derived Stem Cells

1
Department of Oral and Maxillofacial Surgery, Facial Plastic Surgery, University Medical Center Mainz, 55131 Mainz, Germany
2
Department of Oral and Maxillofacial Surgery, Facial Plastic Surgery, University Medical Center Rostock, 18057 Rostock, Germany
3
Institute of Applied Microelectronics and Computer Engineering, University of Rostock, 18051 Rostock, Germany
4
Department of Orthopedics, University Medical Center Rostock, 18057 Rostock, Germany
5
Department of Cardiac Surgery, University Medical Center Rostock, 18059 Rostock, Germany
6
Department Life, Light & Matter (LL&M), University of Rostock, 18059 Rostock, Germany
*
Author to whom correspondence should be addressed.
Biomedicines 2020, 8(11), 482; https://doi.org/10.3390/biomedicines8110482
Received: 15 October 2020 / Revised: 2 November 2020 / Accepted: 6 November 2020 / Published: 8 November 2020
(This article belongs to the Special Issue Soft and Hard Tissue Regeneration)
The aim of the study was to establish electrical stimulation parameters in order to improve cell growth and viability of human adipose-derived stem cells (hADSC) when compared to non-stimulated cells in vitro. hADSC were exposed to continuous electrical stimulation with 1.7 V AC/20 Hz. After 24, 72 h and 7 days, cell number, cellular surface coverage and cell proliferation were assessed. In addition, cell cycle analysis was carried out after 3 and 7 days. After 24 h, no significant alterations were observed for stimulated cells. At day 3, stimulated cells showed a 4.5-fold increase in cell numbers, a 2.7-fold increase in cellular surface coverage and a significantly increased proliferation. Via cell cycle analysis, a significant increase in the G2/M phase was monitored for stimulated cells. Contrastingly, after 7 days, the non-stimulated group exhibited a 11-fold increase in cell numbers and a 4-fold increase in cellular surface coverage as well as a significant increase in cell proliferation. Moreover, the stimulated cells displayed a shift to the G1 and sub-G1 phase, indicating for metabolic arrest and apoptosis initiation. In accordance, continuous electrical stimulation of hADSC led to a significantly increased cell growth and proliferation after 3 days. However, longer stimulation periods such as 7 days caused an opposite result indicating initiation of apoptosis. View Full-Text
Keywords: tissue engineering; human adipose-derived stem cells; proliferation; electrical stimulation; continuous stimulation; alternating current tissue engineering; human adipose-derived stem cells; proliferation; electrical stimulation; continuous stimulation; alternating current
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MDPI and ACS Style

Kämmerer, P.W.; Engel, V.; Plocksties, F.; Jonitz-Heincke, A.; Timmermann, D.; Engel, N.; Frerich, B.; Bader, R.; Thiem, D.G.E.; Skorska, A.; David, R.; Al-Nawas, B.; Dau, M. Continuous Electrical Stimulation Affects Initial Growth and Proliferation of Adipose-Derived Stem Cells. Biomedicines 2020, 8, 482.

AMA Style

Kämmerer PW, Engel V, Plocksties F, Jonitz-Heincke A, Timmermann D, Engel N, Frerich B, Bader R, Thiem DGE, Skorska A, David R, Al-Nawas B, Dau M. Continuous Electrical Stimulation Affects Initial Growth and Proliferation of Adipose-Derived Stem Cells. Biomedicines. 2020; 8(11):482.

Chicago/Turabian Style

Kämmerer, Peer W.; Engel, Vivien; Plocksties, Franz; Jonitz-Heincke, Anika; Timmermann, Dirk; Engel, Nadja; Frerich, Bernhard; Bader, Rainer; Thiem, Daniel G.E.; Skorska, Anna; David, Robert; Al-Nawas, Bilal; Dau, Michael. 2020. "Continuous Electrical Stimulation Affects Initial Growth and Proliferation of Adipose-Derived Stem Cells" Biomedicines 8, no. 11: 482.

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