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Current Status of In Vitro Models and Assays for Susceptibility Testing for Wound Biofilm Infections

1
Division of Molecular Pharmaceutics and Drug Delivery, College of Pharmacy, The University of Texas at Austin, Austin, TX 78712, USA
2
Department of Biomedical Engineering, Cockrell Scholl of Engineering, The University of Texas at Austin, Austin, TX 78712, USA
3
The LaMontagne Center for Infectious Disease, The University of Texas at Austin, Austin, TX 78712, USA
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Biomedicines 2019, 7(2), 34; https://doi.org/10.3390/biomedicines7020034
Received: 30 March 2019 / Revised: 20 April 2019 / Accepted: 26 April 2019 / Published: 30 April 2019
(This article belongs to the Special Issue Biofilms in Wounds: New Advances in Therapy and in Healing Management)
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Abstract

Biofilm infections have gained recognition as an important therapeutic challenge in the last several decades due to their relationship with the chronicity of infectious diseases. Studies of novel therapeutic treatments targeting infections require the development and use of models to mimic the formation and characteristics of biofilms within host tissues. Due to the diversity of reported in vitro models and lack of consensus, this review aims to provide a summary of in vitro models currently used in research. In particular, we review the various reported in vitro models of Pseudomonas aeruginosa biofilms due to its high clinical impact in chronic wounds and in other chronic infections. We assess advances in in vitro models that incorporate relevant multispecies biofilms found in infected wounds, such as P. aeruginosa with Staphylococcus aureus, and additional elements such as mammalian cells, simulating fluids, and tissue explants in an attempt to better represent the physiological conditions found at an infection site. It is hoped this review will aid researchers in the field to make appropriate choices in their proposed studies with regards to in vitro models and methods. View Full-Text
Keywords: biofilms; chronic infections; in vitro models; viability assays biofilms; chronic infections; in vitro models; viability assays
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
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Bahamondez-Canas, T.F.; Heersema, L.A.; Smyth, H.D.C. Current Status of In Vitro Models and Assays for Susceptibility Testing for Wound Biofilm Infections. Biomedicines 2019, 7, 34.

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