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Zebrafish Larvae as a Behavioral Model in Neuropharmacology

Department of Molecular and Translational Medicine, University of Brescia, 25123 Brescia, Italy
Clinical Chemistry Laboratory, ASST-Spedali Civili di Brescia, 25123 Brescia, Italy
Author to whom correspondence should be addressed.
Biomedicines 2019, 7(1), 23;
Received: 12 February 2019 / Revised: 22 March 2019 / Accepted: 22 March 2019 / Published: 26 March 2019
(This article belongs to the Special Issue Zebrafish Models for Development and Disease)
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Zebrafish larvae show a clear and distinct pattern of swimming in response to light and dark conditions, following the development of a swim bladder at 4 days post fertilization. This swimming behavior is increasingly employed in the screening of neuroactive drugs. The recent emergence of high-throughput techniques for the automatic tracking of zebrafish larvae has further allowed an objective and efficient way of finding subtle behavioral changes that could go unnoticed during manual observations. This review highlights the use of zebrafish larvae as a high-throughput behavioral model for the screening of neuroactive compounds. We describe, in brief, the behavior repertoire of zebrafish larvae. Then, we focus on the utilization of light-dark locomotion test in identifying and screening of neuroactive compounds. View Full-Text
Keywords: zebrafish larvae; behavior; neuropharmacology; high-throughput screening; neuroactive drugs; light-dark test zebrafish larvae; behavior; neuropharmacology; high-throughput screening; neuroactive drugs; light-dark test

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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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MDPI and ACS Style

Basnet, R.M.; Zizioli, D.; Taweedet, S.; Finazzi, D.; Memo, M. Zebrafish Larvae as a Behavioral Model in Neuropharmacology. Biomedicines 2019, 7, 23.

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