Transcriptional Regulation and Function of Malic Enzyme 1 in Human Macrophage Activation

Santarsiero, Anna; Todisco, Simona; Convertini, Paolo; De Leonibus, Chiara; Infantino, Vittoria

doi:10.3390/biomedicines12092089

Open AccessArticle

Transcriptional Regulation and Function of Malic Enzyme 1 in Human Macrophage Activation

by

Anna Santarsiero

¹

,

Simona Todisco

¹,

Paolo Convertini

¹,

Chiara De Leonibus

^2,3 and

Vittoria Infantino

^2,*

¹

Department of Science, University of Basilicata, 85100 Potenza, Italy

²

Department of Health Sciences, University of Basilicata, 85100 Potenza, Italy

³

Telethon Institute of Genetics and Medicine (TIGEM), 80078 Pozzuoli, Italy

^*

Author to whom correspondence should be addressed.

Biomedicines 2024, 12(9), 2089; https://doi.org/10.3390/biomedicines12092089

Submission received: 1 August 2024 / Revised: 4 September 2024 / Accepted: 10 September 2024 / Published: 13 September 2024

(This article belongs to the Special Issue Signaling Networks and Gene Expression in Immune Cells)

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Abstract

Macrophages represent primary players of the innate immune system. Macrophage activation triggers several signaling pathways and is tightly associated with metabolic changes, which drive different immune subsets. Recent studies unveil the role of various metabolic enzymes in macrophage activation. Here, we show that malic enzyme 1 (ME1) is overexpressed in LPS-induced macrophages. Through chromatin immunoprecipitation, we demonstrate that ME1 transcriptional regulation is under control of NF-κB. Furthermore, ME1 activity is also increased in activated human PBMC-derived macrophages. Notably, ME1 gene silencing decreases nitric oxide as well as reactive oxygen species and prostaglandin E2 inflammatory mediators. Therefore, modulating ME1 provides a potential approach for immunometabolic regulation and in turn macrophage function.

Keywords: malic enzyme 1; gene expression; NF-κB; macrophage activation; inflammation; immunometabolism; reactive oxygen species; nitric oxide; PGE2

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MDPI and ACS Style

Santarsiero, A.; Todisco, S.; Convertini, P.; De Leonibus, C.; Infantino, V. Transcriptional Regulation and Function of Malic Enzyme 1 in Human Macrophage Activation. Biomedicines 2024, 12, 2089. https://doi.org/10.3390/biomedicines12092089

AMA Style

Santarsiero A, Todisco S, Convertini P, De Leonibus C, Infantino V. Transcriptional Regulation and Function of Malic Enzyme 1 in Human Macrophage Activation. Biomedicines. 2024; 12(9):2089. https://doi.org/10.3390/biomedicines12092089

Chicago/Turabian Style

Santarsiero, Anna, Simona Todisco, Paolo Convertini, Chiara De Leonibus, and Vittoria Infantino. 2024. "Transcriptional Regulation and Function of Malic Enzyme 1 in Human Macrophage Activation" Biomedicines 12, no. 9: 2089. https://doi.org/10.3390/biomedicines12092089

APA Style

Santarsiero, A., Todisco, S., Convertini, P., De Leonibus, C., & Infantino, V. (2024). Transcriptional Regulation and Function of Malic Enzyme 1 in Human Macrophage Activation. Biomedicines, 12(9), 2089. https://doi.org/10.3390/biomedicines12092089

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

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Transcriptional Regulation and Function of Malic Enzyme 1 in Human Macrophage Activation

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