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Volume 11
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10.3390/biomedicines11051321
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Article

Disturbances in Switching between Canonical and Non-Canonical Wnt Signaling Characterize Developing and Postnatal Kidneys of Dab1−/− (yotari) Mice

by
Ilija Perutina
1,
Nela Kelam
2,3,
Mirko Maglica
1,
Anita Racetin
2,3,
Marin Ogorevc
2,
Natalija Filipović
1,2,
Yu Katsuyama
4,
Josip Mišković
1 and
Katarina Vukojević
1,2,3,5,*
1
Department of Anatomy, School of Medicine, University of Mostar, 88000 Mostar, Bosnia and Herzegovina
2
Department of Anatomy, Histology and Embryology, University of Split School of Medicine, 21000 Split, Croatia
3
Department of Medical Genetics, School of Medicine, University of Mostar, 88000 Mostar, Bosnia and Herzegovina
4
Department of Anatomy, Shiga University of Medical Science, Otsu 520-2192, Japan
5
Center for Translational Research in Biomedicine, University of Split School of Medicine, 21000 Split, Croatia
*
Author to whom correspondence should be addressed.
Biomedicines 2023, 11(5), 1321; https://doi.org/10.3390/biomedicines11051321
Submission received: 7 March 2023 / Revised: 11 April 2023 / Accepted: 26 April 2023 / Published: 28 April 2023
(This article belongs to the Special Issue Genetic Research on Kidney Diseases)
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Abstract

This study aims to determine the protein expression patterns of acetylated α-tubulin, inversin, dishevelled-1, Wnt5a/b, and β-catenin in developing (E13.5 and E15.5) and early postnatal (P4 and P14) kidneys of Dab1−/− (yotari) mice, their role in regulating the Wnt signaling pathway, and the possible relation to congenital anomalies of kidney and urinary tract (CAKUT). The analysis of target protein co-expression, observed in the renal vesicles/immature glomeruli, ampullae/collecting ducts, convoluted tubules, metanephric mesenchyme of developing kidneys, but proximal convoluted tubules, distal convoluted tubules and glomeruli of postnatal kidneys, was performed using double immunofluorescence and semi-quantitative methods. The overall expression of acetylated α-tubulin and inversin during normal kidney development increases with higher expression in yotari mice as the kidney acquires mature morphology. An increase in β-catenin and cytosolic DVL-1 levels, indicating a switch from non-canonical to canonical Wnt signaling, is found in the postnatal kidney of yotari mice. In contrast, healthy mouse kidney expresses inversin and Wnt5a/b in the postnatal period, thus activating non-canonical Wnt signaling. Target protein expression patterns in kidney development and the early postnatal period observed in this study could indicate that switching between canonical and non-canonical Wnt signaling is crucial for normal nephrogenesis, while the defective Dab1 gene product in yotari mice may promote CAKUT due to interfering with this process.
Keywords: canonical Wnt signaling; nephrogenesis; α-tubulin; inversin; dishevelled-1; Wnt5a/b; β-catenin; CAKUT; Dab1 canonical Wnt signaling; nephrogenesis; α-tubulin; inversin; dishevelled-1; Wnt5a/b; β-catenin; CAKUT; Dab1

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MDPI and ACS Style

Perutina, I.; Kelam, N.; Maglica, M.; Racetin, A.; Ogorevc, M.; Filipović, N.; Katsuyama, Y.; Mišković, J.; Vukojević, K. Disturbances in Switching between Canonical and Non-Canonical Wnt Signaling Characterize Developing and Postnatal Kidneys of Dab1−/− (yotari) Mice. Biomedicines 2023, 11, 1321. https://doi.org/10.3390/biomedicines11051321

AMA Style

Perutina I, Kelam N, Maglica M, Racetin A, Ogorevc M, Filipović N, Katsuyama Y, Mišković J, Vukojević K. Disturbances in Switching between Canonical and Non-Canonical Wnt Signaling Characterize Developing and Postnatal Kidneys of Dab1−/− (yotari) Mice. Biomedicines. 2023; 11(5):1321. https://doi.org/10.3390/biomedicines11051321

Chicago/Turabian Style

Perutina, Ilija, Nela Kelam, Mirko Maglica, Anita Racetin, Marin Ogorevc, Natalija Filipović, Yu Katsuyama, Josip Mišković, and Katarina Vukojević. 2023. "Disturbances in Switching between Canonical and Non-Canonical Wnt Signaling Characterize Developing and Postnatal Kidneys of Dab1−/− (yotari) Mice" Biomedicines 11, no. 5: 1321. https://doi.org/10.3390/biomedicines11051321

APA Style

Perutina, I., Kelam, N., Maglica, M., Racetin, A., Ogorevc, M., Filipović, N., Katsuyama, Y., Mišković, J., & Vukojević, K. (2023). Disturbances in Switching between Canonical and Non-Canonical Wnt Signaling Characterize Developing and Postnatal Kidneys of Dab1−/− (yotari) Mice. Biomedicines, 11(5), 1321. https://doi.org/10.3390/biomedicines11051321

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