Next Article in Journal
An Insight on Functioning Pancreatic Neuroendocrine Neoplasms
Next Article in Special Issue
Retinal Pigment Epithelium Cell Development: Extrapolating Basic Biology to Stem Cell Research
Previous Article in Journal
Evidence of Improvement of Lower Limb Functioning Using Hydrotherapy on Spinal Cord Injury Patients
Previous Article in Special Issue
Hepatoprotective Efficacy of Cycloastragenol Alleviated the Progression of Liver Fibrosis in Carbon-Tetrachloride-Treated Mice
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
Biomedicines
Volume 11
Issue 2
10.3390/biomedicines11020300
biomedicines-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Article

Biomacromolecular Profile in Human Primary Retinal Pigment Epithelial Cells—A Study of Oxidative Stress and Autophagy by Synchrotron-Based FTIR Microspectroscopy

by
Natasha Josifovska
1,*,
Sofija Andjelic
2,
Lyubomyr Lytvynchuk
3,4,
Xhevat Lumi
2,
Tanja Dučić
5,† and
Goran Petrovski
1,6,†
1
Center for Eye Research and Innovative Diagnostics, Department of Ophthalmology, Oslo University Hospital, and Institute for Clinical Medicine, Faculty of Medicine, University of Oslo, 0450 Oslo, Norway
2
Eye Hospital, University Medical Center, 1000 Ljubljana, Slovenia
3
Department of Ophthalmology, Justus Liebig University, University Hospital Giessen and Marburg GmbH, 35390 Giessen, Germany
4
Karl Landsteiner Institute for Retinal Research and Imaging, 1030 Vienna, Austria
5
CELLS-ALBA, Carrer de la Llum 2-26, Cerdanyola del Valles, 08290 Barcelona, Spain
6
Department of Ophthalmology, University of Split School of Medicine and University Hospital Centre, 21000 Split, Croatia
*
Author to whom correspondence should be addressed.
†
These are shared senior authors.
Biomedicines 2023, 11(2), 300; https://doi.org/10.3390/biomedicines11020300
Submission received: 20 December 2022 / Revised: 12 January 2023 / Accepted: 16 January 2023 / Published: 21 January 2023
(This article belongs to the Special Issue Biomedicines: 10th Anniversary)
Browse Figures
Versions Notes

Abstract

Synchrotron radiation-based Fourier Transform Infrared (SR-FTIR) microspectroscopy is a non-destructive and chemically sensitive technique for the rapid detection of changes in the different components of the cell’s biomacromolecular profile. Reactive oxygen species and oxidative stress may cause damage to the DNA, RNA, and proteins in the retinal pigment epithelium (RPE), which can further lead to age-related macular degeneration (AMD) and visual loss in the elderly. In this study, human primary RPEs (hRPEs) were used to study AMD pathogenesis by using an established in vitro cellular model of the disease. Autophagy—a mechanism of intracellular degradation, which is altered during AMD, was studied in the hRPEs by using the autophagy inducer rapamycin and treated with the autophagy inhibitor bafilomycin A1. In addition, oxidative stress was induced by the hydrogen peroxide (H2O2) treatment of hRPEs. By using SR-FTIR microspectroscopy and multivariate analyses, the changes in the phosphate groups of nucleic acids, Amide I and II of the proteins, the carbonyl groups, and the lipid status in the hRPEs showed a significantly different pattern under oxidative stress/autophagy induction and inhibition. This biomolecular fingerprint can be evaluated in future drug discovery studies affecting autophagy and oxidative stress in AMD.
Keywords: synchrotron-based FTIR microspectroscopy; age-related macular degeneration; human primary RPEs; autophagy; oxidative stress synchrotron-based FTIR microspectroscopy; age-related macular degeneration; human primary RPEs; autophagy; oxidative stress

Share and Cite

MDPI and ACS Style

Josifovska, N.; Andjelic, S.; Lytvynchuk, L.; Lumi, X.; Dučić, T.; Petrovski, G. Biomacromolecular Profile in Human Primary Retinal Pigment Epithelial Cells—A Study of Oxidative Stress and Autophagy by Synchrotron-Based FTIR Microspectroscopy. Biomedicines 2023, 11, 300. https://doi.org/10.3390/biomedicines11020300

AMA Style

Josifovska N, Andjelic S, Lytvynchuk L, Lumi X, Dučić T, Petrovski G. Biomacromolecular Profile in Human Primary Retinal Pigment Epithelial Cells—A Study of Oxidative Stress and Autophagy by Synchrotron-Based FTIR Microspectroscopy. Biomedicines. 2023; 11(2):300. https://doi.org/10.3390/biomedicines11020300

Chicago/Turabian Style

Josifovska, Natasha, Sofija Andjelic, Lyubomyr Lytvynchuk, Xhevat Lumi, Tanja Dučić, and Goran Petrovski. 2023. "Biomacromolecular Profile in Human Primary Retinal Pigment Epithelial Cells—A Study of Oxidative Stress and Autophagy by Synchrotron-Based FTIR Microspectroscopy" Biomedicines 11, no. 2: 300. https://doi.org/10.3390/biomedicines11020300

APA Style

Josifovska, N., Andjelic, S., Lytvynchuk, L., Lumi, X., Dučić, T., & Petrovski, G. (2023). Biomacromolecular Profile in Human Primary Retinal Pigment Epithelial Cells—A Study of Oxidative Stress and Autophagy by Synchrotron-Based FTIR Microspectroscopy. Biomedicines, 11(2), 300. https://doi.org/10.3390/biomedicines11020300

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Metrics

Back to TopTop