Antiproliferative Effects in Colorectal Cancer and Stabilisation in Cyclodextrins of the Phytoalexin Isorhapontigenin
Abstract
:1. Introduction
2. Material and Methods
2.1. Materials
2.2. Equipment and Experimental Procedure
2.2.1. Cell Line and Culture Conditions
2.2.2. Cytotoxicity Test
2.2.3. Fluorimetric Assay
2.2.4. Determination of Stoichiometry and Encapsulation Constants
2.2.5. Determination of Thermodynamic Parameters
2.2.6. Molecular Docking
2.2.7. Water Solubility Measurement
2.2.8. Evaluation of the Stability of the Inclusion Complexes
2.2.9. Data Analysis
3. Results and Discussion
3.1. Inhibition of Cell Proliferation in Human Colorectal Cancer with Isorhapontigenin and Its Analogues
3.2. Determination of the Stoichiometry and Encapsulation Constants of Isorhapontigenin Complexes with Natural and Modified Cyclodextrins
3.3. Influence of pH on the Encapsulation Constants of Isorhapontigenin
3.4. Influence of Temperature on Encapsulation Constants of Isorhapontigenin
3.5. Molecular Docking of Isorhapontigenin Inclusion Complexes with CDs
3.6. Determination of the Improvement in Aqueous Solubility after Molecular Encapsulation
3.7. Time Course Evaluation of the Stability of the Inclusion Complexes
4. Conclusions
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Cyclodextrin | R2 | KF (M−1) | Score | |
---|---|---|---|---|
1:1 Model | 1:2 Model | |||
α-CD | 0.932 | 0.719 | 1265.55 ± 63.28 | −6.9 |
β-CD | 0.709 | 0.467 | 3518.86 ± 175.94 | −9.3 |
γ-CD | - | - | - | −6.6 |
HP-β-CD | 0.992 | 0.925 | 6295.78 ± 314.79 | −10.2 |
M-β-CD | 0.942 | 0.744 | 3823.47 ± 191.17 | −8.9 |
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Navarro-Orcajada, S.; Vidal-Sánchez, F.J.; Conesa, I.; Escribano-Naharro, F.; Matencio, A.; López-Nicolás, J.M. Antiproliferative Effects in Colorectal Cancer and Stabilisation in Cyclodextrins of the Phytoalexin Isorhapontigenin. Biomedicines 2023, 11, 3023. https://doi.org/10.3390/biomedicines11113023
Navarro-Orcajada S, Vidal-Sánchez FJ, Conesa I, Escribano-Naharro F, Matencio A, López-Nicolás JM. Antiproliferative Effects in Colorectal Cancer and Stabilisation in Cyclodextrins of the Phytoalexin Isorhapontigenin. Biomedicines. 2023; 11(11):3023. https://doi.org/10.3390/biomedicines11113023
Chicago/Turabian StyleNavarro-Orcajada, Silvia, Francisco José Vidal-Sánchez, Irene Conesa, Francisco Escribano-Naharro, Adrián Matencio, and José Manuel López-Nicolás. 2023. "Antiproliferative Effects in Colorectal Cancer and Stabilisation in Cyclodextrins of the Phytoalexin Isorhapontigenin" Biomedicines 11, no. 11: 3023. https://doi.org/10.3390/biomedicines11113023