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Volume 10
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Article

A Metabolic Signature to Monitor Endothelial Cell Differentiation, Activation, and Vascular Organization

by
Filipa Lopes-Coelho
1,2,†,
Filipa Martins
1,2,†,
Ana Hipólito
1,2,
Sílvia V. Conde
1,
Sofia A. Pereira
1,
Luís G. Gonçalves
3,‡ and
Jacinta Serpa
1,2,*,‡
1
iNOVA4Health, NOVA Medical School|Faculdade de Ciências Médicas, Universidade NOVA de Lisboa, Campo dos Mártires da Pátria, 130, 1169-056 Lisboa, Portugal
2
Instituto Português de Oncologia de Lisboa Francisco Gentil (IPOLFG), Rua Prof Lima Basto, 1099-023 Lisboa, Portugal
3
Instituto de Tecnologia Química e Tecnológica (ITQB) António Xavier da Universidade Nova de Lisboa, Av. da República, 2780-157 Oeiras, Portugal
*
Author to whom correspondence should be addressed.
†
These authors contributed equally to this work.
‡
These authors contributed equally to this work.
Biomedicines 2022, 10(9), 2293; https://doi.org/10.3390/biomedicines10092293
Submission received: 18 July 2022 / Revised: 9 September 2022 / Accepted: 10 September 2022 / Published: 15 September 2022
(This article belongs to the Special Issue Angiogenesis and Anti-angiogenesis in Health and Diseases)
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Abstract

The formation of new blood vessels is an important step in the morphogenesis and organization of tissues and organs; hence, the success of regenerative medicine procedures is highly dependent on angiogenesis control. Despite the biotechnological advances, tissue engineering is still a challenge. Regarding vascular network formation, the regulators are well known, yet the identification of markers is pivotal in order to improve the monitoring of the differentiation and proliferation of endothelial cells, as well as the establishment of a vascular network supporting tissue viability for an efficacious implantation. The metabolic profile accompanies the physiological stages of cells involved in angiogenesis, being a fruitful hub of biomarkers, whose levels can be easily retrieved. Through NMR spectroscopy, we identified branched amino acids, acetate, and formate as central biomarkers of monocyte-to-endothelial-cell differentiation and endothelial cell proliferation. This study reinforces the successful differentiation process of monocytes into endothelial cells, allowing self-to-self transplantation of patient-derived vascular networks, which is an important step in tissue engineering, since monocytes are easily isolated and autologous transplantation reduces the immune rejection events.
Keywords: endothelial cells; monocytes; differentiation; sprouting; metabolic profile; metabolic signature; biomarkers endothelial cells; monocytes; differentiation; sprouting; metabolic profile; metabolic signature; biomarkers

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MDPI and ACS Style

Lopes-Coelho, F.; Martins, F.; Hipólito, A.; Conde, S.V.; Pereira, S.A.; Gonçalves, L.G.; Serpa, J. A Metabolic Signature to Monitor Endothelial Cell Differentiation, Activation, and Vascular Organization. Biomedicines 2022, 10, 2293. https://doi.org/10.3390/biomedicines10092293

AMA Style

Lopes-Coelho F, Martins F, Hipólito A, Conde SV, Pereira SA, Gonçalves LG, Serpa J. A Metabolic Signature to Monitor Endothelial Cell Differentiation, Activation, and Vascular Organization. Biomedicines. 2022; 10(9):2293. https://doi.org/10.3390/biomedicines10092293

Chicago/Turabian Style

Lopes-Coelho, Filipa, Filipa Martins, Ana Hipólito, Sílvia V. Conde, Sofia A. Pereira, Luís G. Gonçalves, and Jacinta Serpa. 2022. "A Metabolic Signature to Monitor Endothelial Cell Differentiation, Activation, and Vascular Organization" Biomedicines 10, no. 9: 2293. https://doi.org/10.3390/biomedicines10092293

APA Style

Lopes-Coelho, F., Martins, F., Hipólito, A., Conde, S. V., Pereira, S. A., Gonçalves, L. G., & Serpa, J. (2022). A Metabolic Signature to Monitor Endothelial Cell Differentiation, Activation, and Vascular Organization. Biomedicines, 10(9), 2293. https://doi.org/10.3390/biomedicines10092293

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