Next Article in Journal
Molecularly Imprinted Nanoparticles towards MMP9 for Controlling Cardiac ECM after Myocardial Infarction: A Predictive Experimental-Computational Chemistry Investigation
Next Article in Special Issue
“Mens Sana in Corpore Sano”: The Emerging Link of Motor Reserve with Motor and Cognitive Abilities and Compensatory Brain Networks in SCA2 Patients
Previous Article in Journal
Beneficial Effects of Empagliflozin Are Mediated by Reduced Renal Inflammation and Oxidative Stress in Spontaneously Hypertensive Rats Expressing Human C-Reactive Protein
Previous Article in Special Issue
Fluorescent In Situ Staining and Flow Cytometric Procedures as New Pre-Diagnostic Tests for Sialidosis, GM1 Gangliosidosis and Niemann–Pick Type C
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
Biomedicines
Volume 10
Issue 9
10.3390/biomedicines10092069
biomedicines-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Article

Metabolic Profile Variations along the Differentiation of Human-Induced Pluripotent Stem Cells to Dopaminergic Neurons

by
Emma Veronica Carsana
1,†,
Matteo Audano
2,†,
Silvia Breviario
1,†,
Silvia Pedretti
2,
Massimo Aureli
1,*,
Giulia Lunghi
1,‡ and
Nico Mitro
2,*,‡
1
Department of Medical Biotechnology and Translational Medicine, University of Milan, 20054 Milan, Italy
2
Department of Pharmacological and Biomolecular Sciences, University of Milan, 20122 Milan, Italy
*
Authors to whom correspondence should be addressed.
†
These authors contributed equally to this work.
‡
These authors contributed equally to this work.
Biomedicines 2022, 10(9), 2069; https://doi.org/10.3390/biomedicines10092069
Submission received: 27 July 2022 / Revised: 17 August 2022 / Accepted: 20 August 2022 / Published: 24 August 2022
(This article belongs to the Special Issue State of the Art: Neurodegenerative Diseases in Italy)
Browse Figures
Review Reports Versions Notes

Abstract

In recent years, the availability of induced pluripotent stem cell-based neuronal models has opened new perspectives on the study and therapy of neurological diseases such as Parkinson’s disease. In particular, P. Zhang set up a protocol to efficiently generate dopaminergic neurons from induced pluripotent stem cells. Although the differentiation process of these cells has been widely investigated, there is scant information related to the variation in metabolic features during the differentiation process of pluripotent stem cells to mature dopaminergic neurons. For this reason, we analysed the metabolic profile of induced pluripotent stem cells, neuronal precursors and mature neurons by liquid chromatography–tandem mass spectrometry. We found that induced pluripotent stem cells primarily rely on fatty acid beta-oxidation as a fuel source. Upon progression to neuronal progenitors, it was observed that cells began to shut down fatty acid β-oxidation and preferentially catabolised glucose, which is the principal source of energy in fully differentiated neurons. Interestingly, in neuronal precursors, we observed an increase in amino acids that are likely the result of increased uptake or synthesis, while in mature dopaminergic neurons, we also observed an augmented content of those amino acids needed for dopamine synthesis. In summary, our study highlights a metabolic rewiring occurring during the differentiation stages of dopaminergic neurons.
Keywords: iPSCs; dopaminergic neurons; mass spectrometry; metabolism; neuronal differentiation iPSCs; dopaminergic neurons; mass spectrometry; metabolism; neuronal differentiation
Graphical Abstract

Share and Cite

MDPI and ACS Style

Carsana, E.V.; Audano, M.; Breviario, S.; Pedretti, S.; Aureli, M.; Lunghi, G.; Mitro, N. Metabolic Profile Variations along the Differentiation of Human-Induced Pluripotent Stem Cells to Dopaminergic Neurons. Biomedicines 2022, 10, 2069. https://doi.org/10.3390/biomedicines10092069

AMA Style

Carsana EV, Audano M, Breviario S, Pedretti S, Aureli M, Lunghi G, Mitro N. Metabolic Profile Variations along the Differentiation of Human-Induced Pluripotent Stem Cells to Dopaminergic Neurons. Biomedicines. 2022; 10(9):2069. https://doi.org/10.3390/biomedicines10092069

Chicago/Turabian Style

Carsana, Emma Veronica, Matteo Audano, Silvia Breviario, Silvia Pedretti, Massimo Aureli, Giulia Lunghi, and Nico Mitro. 2022. "Metabolic Profile Variations along the Differentiation of Human-Induced Pluripotent Stem Cells to Dopaminergic Neurons" Biomedicines 10, no. 9: 2069. https://doi.org/10.3390/biomedicines10092069

APA Style

Carsana, E. V., Audano, M., Breviario, S., Pedretti, S., Aureli, M., Lunghi, G., & Mitro, N. (2022). Metabolic Profile Variations along the Differentiation of Human-Induced Pluripotent Stem Cells to Dopaminergic Neurons. Biomedicines, 10(9), 2069. https://doi.org/10.3390/biomedicines10092069

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Metrics

Back to TopTop