Mechanisms of Synaptic Vesicle Exo- and Endocytosis

Round 1

Reviewer 1 Report

-This review article makes a nice contribution in summarizing the existing knowledge in synaptic vesicle exocytosis and vesicle recovery by endocytosis. 

-It would be more informative if Fig. 1 showed the order of events of vesicle tethering, docking and fusion.

-The resolution of Fig 1 is low (it is too pixelated).

-In synaptic vesicle exocytosis, the author takes into account only the role of Rab3, while it is known that Rab27b is also involved, which should be included in the review.

-Coordination between endocytosis and exocytosis should be shown by a figure that describes the involved molecular players.

-As the article uses numerus abbreviations, a list of them, with their explanation, is required.

-The manuscript requires editing, to improve the flow of the language and to make it easier for the reader to follow.

Author Response

Reviewer 1

-This review article makes a nice contribution in summarizing the existing knowledge in synaptic vesicle exocytosis and vesicle recovery by endocytosis. 

I am relieved to know that the topics in my review are suitable for the special issue. 

-It would be more informative if Fig. 1 showed the order of events of vesicle tethering, docking and fusion.

I am very sorry that I have no good tool (application) to produce a drawing suggested by the reviewer 1.  So, I added a sentence at the last of the figure legends, “Detailed order of events of vesicle tethering, docking and fusion, please refer reviews [11, 39].”

-The resolution of Fig 1 is low (it is too pixelated).

The Fig.1 is replaced by Figure 1 with better resolution.

-In synaptic vesicle exocytosis, the author takes into account only the role of Rab3, while it is known that Rab27b is also involved, which should be included in the review.

Rab27 and references are added to the text (line 92 and 93) and to the Figure 1.

-Coordination between endocytosis and exocytosis should be shown by a figure that describes the involved molecular players.

I added Figure 2 which shows linkage of endocytosis and exocytosis mediated by possible molecular players.

-As the article uses numerus abbreviations, a list of them, with their explanation, is required.

I added the list of abbreviations at the last of text.

-The manuscript requires editing, to improve the flow of the language and to make it easier for the reader to follow.

I will ask the editor of the Biomedicines.

Reviewer 2 Report

The purpose of the authors in this manuscript is summarizing Ca2⁺-dependent protein controls of synchronous and asynchronous vesicle release, rapid replenishment of the active zone, endocytosis, and short-term plasticity within 100 msec after the action potential. This article not only provides a very comprehensive summary of the mechanism, but also provides an outlook on the future of this research direction.

The manuscript is well written. Only suggest the author to add a table to better summarize the article. This manuscript met the goal of Biomedicines.

Author Response

Reviewer 2: Only suggest the author to add a table to better summarize the article. This manuscript met the goal of Biomedicines.

I added a table of protein function in the presynaptic terminal following contents of the text.