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Article

Retrograde Analysis of Calcium Signaling by CaMPARI2 Shows Cytosolic Calcium in Chondrocytes Is Unaffected by Parabolic Flights

1
Institute for Molecular Biosciences, Johann Wolfgang Goethe Universität, 60438 Frankfurt am Main, Germany
2
Space Biology Group, Institute of Medical Engineering, School of Engineering and Architecture, Lucerne University of Applied Sciences and Arts, 6052 Hergiswil, Switzerland
3
National Center for Biomedical Research in Space, University of Zurich, Innovation Cluster Space and Aviation (UZH Space Hub), 8600 Dübendorf, Switzerland
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Institute of Mechanical Engineering and Energy Technology, School of Engineering and Architecture, Lucerne University of Applied Sciences and Arts, 6048 Horw, Switzerland
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Institute of Electrical Engineering, School of Engineering and Architecture, Lucerne University of Applied Sciences and Arts, 6048 Horw, Switzerland
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Buchmann Institute for Molecular Life Sciences (BMLS), Johann Wolfgang Goethe Universität, 60438 Frankfurt am Main, Germany
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Institute of Biochemistry 2, Johann Wolfgang Goethe Universität, 60438 Frankfurt am Main, Germany
*
Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
Academic Editors: Daniela Grimm and Ruth Hemmersbach
Biomedicines 2022, 10(1), 138; https://doi.org/10.3390/biomedicines10010138
Received: 23 December 2021 / Revised: 5 January 2022 / Accepted: 5 January 2022 / Published: 8 January 2022
Calcium (Ca2+) elevation is an essential secondary messenger in many cellular processes, including disease progression and adaptation to external stimuli, e.g., gravitational load. Therefore, mapping and quantifying Ca2+ signaling with a high spatiotemporal resolution is a key challenge. However, particularly on microgravity platforms, experiment time is limited, allowing only a small number of replicates. Furthermore, experiment hardware is exposed to changes in gravity levels, causing experimental artifacts unless appropriately controlled. We introduce a new experimental setup based on the fluorescent Ca2+ reporter CaMPARI2, onboard LED arrays, and subsequent microscopic analysis on the ground. This setup allows for higher throughput and accuracy due to its retrograde nature. The excellent performance of CaMPARI2 was demonstrated with human chondrocytes during the 75th ESA parabolic flight campaign. CaMPARI2 revealed a strong Ca2+ response triggered by histamine but was not affected by the alternating gravitational load of a parabolic flight. View Full-Text
Keywords: CaMPARI; articular chondrocytes; cytosolic free calcium; gravity; parabolic flight; high throughput screening CaMPARI; articular chondrocytes; cytosolic free calcium; gravity; parabolic flight; high throughput screening
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MDPI and ACS Style

Hammer, A.; Cerretti, G.; Ricciardi, D.A.; Schiffmann, D.; Maranda, S.; Kummer, R.; Zumbühl, C.; Rattenbacher-Kiser, K.F.; von Arx, S.; Ammann, S.; Strobl, F.; Berkane, R.; Stolz, A.; Stelzer, E.H.K.; Egli, M.; Schleiff, E.; Wuest, S.L.; Böhmer, M. Retrograde Analysis of Calcium Signaling by CaMPARI2 Shows Cytosolic Calcium in Chondrocytes Is Unaffected by Parabolic Flights. Biomedicines 2022, 10, 138. https://doi.org/10.3390/biomedicines10010138

AMA Style

Hammer A, Cerretti G, Ricciardi DA, Schiffmann D, Maranda S, Kummer R, Zumbühl C, Rattenbacher-Kiser KF, von Arx S, Ammann S, Strobl F, Berkane R, Stolz A, Stelzer EHK, Egli M, Schleiff E, Wuest SL, Böhmer M. Retrograde Analysis of Calcium Signaling by CaMPARI2 Shows Cytosolic Calcium in Chondrocytes Is Unaffected by Parabolic Flights. Biomedicines. 2022; 10(1):138. https://doi.org/10.3390/biomedicines10010138

Chicago/Turabian Style

Hammer, Andreas, Geraldine Cerretti, Dario A. Ricciardi, David Schiffmann, Simon Maranda, Raphael Kummer, Christoph Zumbühl, Karin F. Rattenbacher-Kiser, Silvan von Arx, Sebastian Ammann, Frederic Strobl, Rayene Berkane, Alexandra Stolz, Ernst H. K. Stelzer, Marcel Egli, Enrico Schleiff, Simon L. Wuest, and Maik Böhmer. 2022. "Retrograde Analysis of Calcium Signaling by CaMPARI2 Shows Cytosolic Calcium in Chondrocytes Is Unaffected by Parabolic Flights" Biomedicines 10, no. 1: 138. https://doi.org/10.3390/biomedicines10010138

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