All-Solid-State Calcium Sensors Modified with Polypyrrol (PPY) and Graphene Oxide (GO) as Solid-Contact Ion-to-Electron Transducers

Abstract

Reliable, cost-effective, and robust screen-printed sensors were constructed and presented for Ca²⁺ ions determination. The sensors were based on the use of bilirubin (1,3,6,7-tetramethyl-4,5- dicarboxyethy-2,8-divinyl-(b-13)-dihydrobilenone) as a recognition sensory material in plasticized poly (vinyl chloride) (PVC) membranes. Polypyrrol (PPY) and graphene oxide (GO) were used as ion-to-electron transducers, where the effects of anionic excluder, pH, and selectivity were investigated. In a 50 mM tris buffer solution of pH 5, the electrodes offered a potential response for Ca²⁺ ions with a near-Nernstian slopes of 38.1 ± 0.4 (r² = 0.996) and 31.1 ± 0.6 (r² = 0.999), detection limits 3.8 × 10⁻⁶ (0.152 μg/mL) and 2.3 × 10⁻⁷ M (8.0 ng/mL), and linear concentration ranges of 7.0 × 10⁻⁶–1.0 × 10⁻² (400–0.28 μg/mL) and 7.0 × 10⁻⁷–1.0 × 10⁻² M (400–0.028 μg/mL) for sensors based on PPY and GO, respectively. Both sensors revealed stable potentiometric responses with excellent reproducibility and enhanced selectivity over a number of most common metal ions, such as Na⁺, K⁺, Li⁺, NH₄⁺, Fe²⁺, Mg²⁺, and Ba²⁺. Impedance spectroscopy and chronopotentiometric techniques were used for evaluating the potential drift and the interfacial sensor capacitance. The proposed sensors offered the advantages of simple design, ability of miniaturization, good potential stability, and cost-effectiveness. The developed electrodes were applied successfully to Ca²⁺ ion assessment in different pharmaceutical products, baby-food formulations, and human blood samples. The results obtained were compared with data obtained by atomic absorption spectrometry (AAS).

1. Introduction

Calcium is an important element that plays an essential physiological role in the human body, along with Na⁺, K⁺, Cl^-, and Mg²⁺. It is mandatory for bone formation that plays the role of support and protection of the human body. Calcium is necessary for cell secretion, signal transmission, chemical, and electrical stimulation for neuromuscular transmission, as well as blood clotting [1]. Therefore, the calcium assessment in human blood is essential in the medical field within intensive care units [2], in open-heart surgeries and cardiovascular treatments [3], and in organ transplants [4].

Calcium sensors based on potentiometric transduction have been developed several decades ago and were pioneered by Ross [5]. The measurement of ionized calcium in environmental, industrial, and clinical blood samples require reliable, sensitive, rapid, and cost-effective methods [6,7]. Of all the electrochemical techniques, potentiometry is probably the most preferred technique that fulfills all of these advantages. Potentiometric sensors “ion-selective electrodes” (ISEs), in a short time frame, can provide sensitive and reliable measurements for clinically critical analyses of small sample sizes [8,9,10]. In addition, the measuring devices for potentiometric sensors have the advantages of small size, simplicity of use, and provide fast and reliable responses.

There is an increasing importance to realize compact sensing devices. Therefore, the design and manufacture of solid-state reference electrodes that are easy to miniaturize and the cost-effectiveness of their mass production are becoming increasingly important to achieve this purpose. Recently, there has been an increasing and accelerating demand for wearable sensors and point-of-care tests that provide real-time information on health standards, and many of these systems are operated electrochemically. The all-solid-state ISEs, especially, are attractive for wide range of practical applications because they are liquid junction free, pressure resistant, easy to pack and seal, and allow for the miniaturization for portable devices [11,12,13,14]. To fabricate these types of ISEs, it requires careful design of a solid electronic conductor and solid-contact material, due to their mixed ionic and electronic conductivity through solution-casting [15]. Different solid electronic conductors were investigated in the fabrication of solid-contact ISEs, such as gold [16,17], platinum [18,19], silicon [20], graphite/epoxy [21], and glassy carbon [22]. These materials are suffering from either the high cost or complication in the fabrication process. All solid-planar electrodes are based on electrodes made of an electronic solid-conductor coated by the ion-sensing membrane (ISM) and a conducting solid-contact layer is inserted [23]. The processing of thick films is effective for manufacturing compact and easily reproducible structural sensors at a modest cost [24] and leads to the feasibility of mass production of portable disposable sensors for point of care testing [25].

Bilirubin consists of an open chain tetrapyrrole. It is formed by the oxidative cleavage of porphyrin in heme, which affords biliverdin. Its structure has four methyl groups, two propionic acid groups, and two vinyl radicals. Bilirubin has a great affinity towards Ca²⁺ forming Ca(II)-bilirubinate complex, in which four nitrogen atoms of bilirubin surround a Ca(II) ions in a quasi-square planer arrangement [26,27].

In this work, we report new screen-printed calcium sensors based on bilirubin as a recognition sensory material and tetrakis-(p-chlorophenyl) borate as a lipophilic anion. The plasticized PVC membrane was coated on either polypyrrol (PPY) or graphene oxide (GO) as solid contact transducers. Performance characteristics of the proposed sensors, including sensitivity, selectivity, pH effect, and potential stability were studied. The capacitative character of solid contact transducers was evaluated using electrochemical impedance spectroscopy (EIS) and chronopotentiometric techniques. The sensors were successfully applied for monitoring Ca²⁺ ions in some pharmaceutical formulations, baby-food products, and human serum plasma.

2. Experimental

2.1. Apparatus

Screen-printed carbon electrodes (SPCEs) modified with a monolayer of either polypyrrol (PPY) (Ref. 110PPYR) or graphene oxide (GO) (Ref. 110GPHOX) were purchased from DropSens (LLanera, Asturias, Spain). The platforms were of ceramic substrate (L34 × W10 × H0.5 mm) and silver as an electrical contact. The electrochemical cell consists of either GO/carbon or PPY/carbon (4 mm) acting as a working electrode and Ag/AgCl as a reference electrode. All potentiometric measurements were carried out at ambient temperature using a pH/mV ion meter (PXSJ-216 INESA Scientific Instrument Co., Ltd, Shangahi, China). Electrochemical impedance spectroscopy (EIS) and chronopotentiometry data were obtained using a potentiostat/galvanostat (Metrohom, Autolab 204, Herisau, Switzerland).

2.2. Materials and Reagents

Bilirubin, high molecular weight poly (vinyl chloride) (PVC), (o-nitrophenyloctyl ether (o-NPOE), and tetrahydrofuran (THF) were obtained from Sigma (St. Louis, MO, USA). Potassium tetrakis (4-chlorophenyl) borate (KTClPB) was purchased from Fluka AG (Buchs, Switzerland). All other chemicals were of analytical grade quality.

A stock 10⁻¹ M solution of Ca²⁺ was prepared by dissolving anhydrous CaCl₂ salt in de-ionized water and then standardized via its titration with EDTA solution.

2.3. Sensors’ Construction

Plasticized poly (vinyl chloride) (PVC) membrane sensors were fabricated using o-NPOE plasticizer in the presence of KTClPB as an anionic excluder. The composition of the membrane consisted of 3.0 wt% bilirubin ionophore (3.0 mg), 1.5 wt% KTClPB (1.5 mg), 31.5 wt% PVC (31.5 mg), and 63.5 wt% o-NPOE (63.5 mg). All were dissolved in 2 mL THF solvent. Subsequently, 100 µL of the membrane cocktail was drop-casted over the transducer layer (PPY for sensor I or GO for sensor II) coating the carbon conductor in the screen-printed electrode. The sensors were stored in dry place for 4 hours, then soaked in 10⁻⁴ M Ca²⁺ solution for one day, followed by another two days in a 10⁻⁷ M Ca²⁺ ion solution.

2.4. Electrochemical Impedance Spectroscopy and Chronopotentiometry

Electrochemical impedance spectroscopy (EIS) and chronopotentiometry measurements were carried out in 10⁻⁴ M CaCl₂ solution using a conventional three-electrode system, where the studied sensor was connected as the working electrode. The reference electrode of the system was Ag/AgCl (3 M KCl), and the auxiliary electrode was made from a platinum wire. The impedance spectra were recorded at open circuit potential with an amplitude 100 mV and in the frequency range 0.1 Hz–100 kHz. The reversed-current chronopotentiometry was carried out after applying a constant current of a value ±1 nA for 120 s.

2.5. Calcium Assessment in Real Samples

The proposed sensors were used for calcium determination in some baby-food products, human serum, and some pharmaceutical formulations. For baby-food samples, a 1.0 g sample was mixed with 10 mL of (1:1) HNO₃ in a silica crucible, and the mixture was heated till dryness. To the obtained residue, a 5 mL volume of 0.1 M HCl was added and the mixture was then heated for further 1 h. The solution was filtered and collected in a 50 mL volumetric flask, then completed to the mark with 50 mM tris buffer (pH = 5). This solution was stored in a brown bottle and analyzed using the proposed sensors, in comparison with the atomic absorption spectroscopy (AAS) reference method.

Calcium is assessed by oral dosage of the pharmaceutical products labeled commercially as Calcimate (500 mg/tablet) (ADWIC, Oubour, Qalyubia, Egypt) and Vitacid calcium (800 mg/tablet) (CID, Talbeyah Al Qebleyah, El Talbia, Giza Governorate, Egypt). Three tablets of each formulation containing calcium were grinded into a homogeneous fine powder using an agate mortar. A definite amount of the mixed finely powdered 3 tablets, equivalent to one tablet, was accurately transferred into a 50 mL beaker and 5 mL of (1:1) HCl added. The reaction mixture was then sonicated for 10 min and heated for a further 1 h at 80 °C. The solution was transferred after cooling into a 100 mL calibrated flask and completed to the mark with 50 mM tris buffer (pH = 5). Potential measurements of sample solutions were recorded and Ca²⁺ ion concentrations were calculated using the standard calibration curve method.

For calcium determination in human serum, blood samples were acquired from healthy humans with informed consent and then stored refrigerated for 8 hrs before extracting its serum. Serum was extracted after centrifugation at 12,000 rpm for 20 min. A 2.0 mL sample of clear blood serum is transferred into a 250 mL calibrated flask. The sample is diluted to 50 mL with de-ionized water. Then, 9 mL of 50 mM tris buffer solution of pH 5 was added. The mixture was mixed and used for calcium measurements. The sensors were immersed in the sample solution and the potential values were plotted versus log (Ca²⁺) concentration to construct the calibration plot. The study protocol was approved by the National Research Centre Medical Research Ethics Committee (HU/IACUC20187).

3. Results and Discussion

3.1. Performance Characteristics of the Proposed Sensors

The performance characteristics of the proposed sensors, in terms of working concentration range, detection limit, slope sensitivity and response time were evaluated and determined. All data obtained from the potentiometric measurements were summarized in

Table 1. Potentiometric characteristics of SPC/Ca²⁺-ISEs in 50 mM, tris buffer solution (pH = 5).

Parameter	C/PPY/Ca2+-ISEs	C/GO/Ca2+-ISEs	C/Ca2+-ISEs
Slope (mV/decade)	38.1 ± 0.4	31.1 ± 0.6	34.7 ± 0.3
Correlation coefficient (r2)	0.996	0.999	0.995
Linear range (M)	7.0 × 10−6–1.0 × 10−2	7.0 × 10−7–1.0 × 10−2	1.0 × 10−5–1.0 × 10−2
Detection limit (M)	3.8 × 10−6	2.3 × 10−7	8.0 × 10−6
Working acidity range (pH)	4.0–7.4	3.5–8.4	3.5–8.4
Response time (s)	<5	<5	<5
Accuracy (%)	99.1	99.4	98.8
Precision (%)	1.3	0.7	1.1
Trueness (%)	99.2	99.3	98.7
Bias (%)	0.6	0.4	0.9
Within-day repeatability (%)	0.8	0.5	1.1
Between-days variations (%)	1.1	0.8	1.2

. As shown in the constructed calibration curve (Figure 1), the developed C/PPY/Ca²⁺-ISEs and C/GO/Ca²⁺-ISEs revealed linear ranges of 7.0 × 10⁻⁶–1.0 × 10⁻² and 7.0 × 10⁻⁷–1.0 × 10⁻² M with Nernstian slopes of 38.1 ± 0.4 (r² = 0.996) and 31.1 ± 0.6 (r² = 0.999) mV/decade, respectively. The detection limits for the two sensors were found to be 3.8 × 10⁻⁶ and 2.3 × 10⁻⁷ M, respectively. The un-modified C/Ca²⁺-ISE revealed a near-Nernstian slope of 34.7 ± 0.3 mV/decade over the linear range of 1.0 × 10⁻⁵–1.0 × 10⁻² M and a detection limit of 8.0 × 10⁻⁶ M. The chemical structure of both bilirubin and calcium bilirubinate complex, in addition to a scheme for electrode response mechanism, is presented in Figure 2.

The response time for the proposed electrodes was evaluated after successive immersion of the sensors in ascending Ca²⁺ ion concentrations. Within the concentration range 1.0 × 10⁻⁷ to 1.0 × 10⁻² M, the time required to achieve a 95% of the equilibrium potential was found to be less than 10 s for all proposed sensors as shown in Figure 1. Relative standard deviations of 2.5% and 3.1% (n = 5) are calculated for full reversible potential response obtained by both C/PPY/Ca²⁺-ISEs and C/GO/Ca²⁺-ISEs, respectively.

The pH effect on the potential response of the developed electrodes was examined over the pH range 2 to 10 for 1.0 × 10⁻⁴ M Ca²⁺ solutions using hydrochloric acid/sodium hydroxide solutions. As shown in Figure 3, all sensors exhibited a stable potential response over the pH range 3.5 to 8.4. At pH > 8.5, the potential begins to decline due to the formation of the detectable Ca(OH)₂ species. Below pH < 3.5, the potential response is decreased, which can result from the dissociation of the Ca(II)-bilirubinate complex. All subsequent measurements were carried out in 50 mM, tris buffer solution of pH 5, which is suitable for the formation of Ca-bilirubinate complex [26].

One of the most important performance characteristics in ion-selective electrodes (ISEs) is its selectivity towards the primary ion over other ions. Selectivity of both C/PPY/Ca²⁺-ISEs and C/GO/Ca²⁺-ISEs was examined using the method proposed by Bakker modified (i.e., separate solution method (MSSM)) (by extrapolating the response functions to a_j = 1 M) [28]. All selectivity coefficient values obtained for the C/PPY/Ca²⁺-ISEs and C/GO/Ca²⁺-ISEs were summarized in

Table 2. Potentiometric selectivity coefficient Log K^pot_Ca²⁺_,J for the proposed calcium-ISEs.

Sensor Type	Log KpotCa2+,J
	Na+	K+	NH4+	Mg2+	Ba2+	Fe2+	Li+
C/PPY/Ca2+-ISEs	−4.3 ± 0.3	−3.7 ± 0.5	−3.9 ± 0.8	−5.1 ± 0.3	−5.8 ± 0.2	−3.9 ± 0.4	−6.3 ± 0.2
C/GO/Ca2+-ISEs	−4.4 ± 0.2	−3.8 ± 0.7	−3.8 ± 0.7	−5.3 ± 0.1	−5.7 ± 0.4	−3.5 ± 0.7	−6.1 ± 0.5
C/Ca2+-ISEs	−4.2 ± 0.5	−3.7 ± 0.4	−3.6 ± 0.6	−5.4 ± 0.2	−5.6 ± 0.3	−3.1 ± 0.8	−6.2 ± 0.3

. It can be seen that C/PPY/Ca²⁺-ISEs and C/GO/Ca²⁺-ISEs exhibited high Ca²⁺ selectivity over interfering ions and the selectivity was not affected by the inserted transducer material, but is dependent on the ion-sensing membrane (ISM) itself. This selectivity behavior satisfied the fulfillments required for calcium determination in different matrix samples.

3.2. Chronopotentiometry

Reversed-current chronopotentiometry (±1 nA) was used to determine the short-term potential stability of the C/PPY/Ca²⁺-ISEs and C/GO/Ca²⁺-ISEs. Typical chronopotentiograms for C/Ca²⁺-ISEs, C/PPY/Ca²⁺-ISEs and C/GO/Ca²⁺-ISEs were shown in Figure 4. The potential drifts for the presented electrodes were calculated from the slope (ΔE/Δt) of the potential–time plot. They were found to be 112.2, 18.3, and 11.4 µV/s for C/Ca²⁺-ISEs, C/PPY/Ca²⁺-ISEs, and C/GO/Ca²⁺-ISEs, respectively. It was noticed that the insertion of a solid-contact layer between the ion-sensing membrane (ISM) and the conducting substrate (carbon) enhanced the potential stability of the electrodes as compared to the electrodes with no solid-contact layer (i.e., C/GO/Ca²⁺-ISEs). In addition, GO based electrodes revealed higher potential stability than PPY based electrodes. The low-frequency capacitance values for C/Ca²⁺-ISEs, C/PPY/Ca²⁺-ISEs, and C/GO/Ca²⁺-ISEs were calculated from the equation (ΔE/Δt = I/C) [29], and found to be 8.9 ± 0.8, 54.6 ± 1.1 and 88.4 ± 1.3 µF, respectively. The bulk resistance of the membrane sensors (R_t = ΔE/I) were also calculated and were found to be R_t = 1.22, 0.36 and 0.67 MΏ for C/Ca²⁺-ISEs, C/PPY/Ca²⁺-ISEs, and C/GO/Ca²⁺-ISEs, respectively. A comparison between the response characteristics of previously reported Ca²⁺-ISEs with different ion-to-electron transducers and the sensors reported in this study is presented in

Table 3. Between various functional materials used as solid-contact transducers in Ca²⁺-ISEs.

Ionophore	Functional Materials	Conductive Substrate	Detection Limit, M	Slope, mV/decade	Potential Drift, µV/s	Interfacial Capacitance, µF	Ref.
Calcium ionophore IV (ETH 5234)	Black phosphorus	GC	4.0 × 10−7	28.3 ± 0.7	72	-	[30]
Calcium ionophore II (ETH129)	SWCNTs	Cu	6.3 × 10−7	28.7	930	5.37	[31]
Calcium ionophore II	P-NPEDMA	Au	3.2 × 10−6	30.2 ± 0.5	-	-	[32]
Calcichrome	Polypyrrole	Pt or GC	1.0 × 10−5	29.1 ± 0.8	-	-	[33]
Calcium ionophore IV (ETH 5234)	Polyazulene	Pt/ZnSe	-	-	0.14	874	[34]
Calcium ionophore IV (ETH 5234)	POT	Pt/ZnSe	2.0 × 10−8	27.3 ± 0.1	-	-	[35]
Calcium ionophore I (ETH-1001)	POT	GC	1.0 × 10−6	25.0 ± 0.9	-	-	[36]
Calcium ionophore II (ETH129)	PEDOT/PSS	Carbon	1.0 × 10−4	30.0 ± 1.0	-	-	[37]
Calcium ionophore II (ETH129)	PEDOT/PSS	Pt	1.0 × 10−6	27.7	-	-	[38]
Calcium ionophore I (ETH-1001)	Graphene/Polyaniline	GC	5.0 × 10−8	28.7 ± 0.1	87	11	[39]
Bilirubin	Polypyrrol	Carbon	3.8 × 10−6	38.1 ± 0.4	18.3	54.6	This work
	Graphene oxide		2.3 × 10−7	31.1 ± 0.6	11.4	88.4

P-NPEDMA: n-phenyl-ethylenediamine-methacrylamide; POT: poly (3-octylthiophene); GC: glassy carbon; PEDOT/PSS: poly (3,4-ethylenedioxythiophene) (PEDOT) doped with poly (styrenesulfonate) (PSS).

.

3.3. Electrochemical Impedance Spectroscopy

For further characterization, to study the quality of both GO and PPY as solid contacts, electrochemical impedance spectroscopy (EIS) was carried out in 10⁻³ M CaCl₂ with the frequency range of 1 Hz–10 kHz, E_dc = 0.2 V, and ΔE_dc = 10 mV. The impedance spectra for C/Ca²⁺-ISEs, C/PPY/Ca²⁺-ISEs and C/GO/Ca²⁺-ISEs are shown in Figure 5. Form the high-frequency semicircle, the bulk membrane between the electronic conductor or solid-contact and the ISM can be evaluated. The resistances were found to be 7.6, 51.2, and 83.5 MΏ for C/Ca²⁺-ISEs, C/PPY/Ca²⁺-ISEs, and C/GO/Ca²⁺-ISEs, respectively. This confirms that the charge-transfer across the interfaces is facilitated due to the presence of the solid-contact transducer. The low-frequency semicircle is associated with the interfacial capacitance (i.e., double-layer capacitance in case of GO and redox capacitance in case of PPY). The calculated interfacial capacitances were found to be 3.07, 0.11 and 0.15 µF for C/Ca²⁺-ISEs, C/PPY/Ca²⁺-ISEs, and C/GO/Ca²⁺-ISEs, respectively. The results indicate that the presence of either PPY or GO as solid-contact transducers between the ISM and the electronic conductor substrate increased the low-frequency capacitance of the proposed all-solid-state Ca²⁺-ISE.

3.4. Water-Layer Test

To test the formation of water layer between the ion-sensing membrane and the internal electrode, the electrodes were initially inserted in 10⁻² M CaCl₂ solution. After 12 h, the solution was changed to the discriminating ion solution (0.1 M NaCl). After 4 h, the discriminating ion solution was replaced by 10⁻² M CaCl₂ solution. The potential readings vs. time were plotted and presented in Figure 6. Both C/PPY/Ca²⁺-ISEs and C/GO/Ca²⁺-ISEs showed very stable potentials upon replacing the primary ions by the discriminated ions while C/Ca²⁺-ISEs exhibited a significant potential drift. This can confirm that, when the hydrophobic solid-contact materials are inserted between the ion-sensing membrane and electronic conductor, no aqueous layer will be formed, and a stable electrode potential will be obtained.

3.5. Analytical Applications

To verify the successful application of the proposed sensors, these electrodes were used for calcium ion determination in different matrices such as some baby-food products, pharmaceutical formulations, and human serum samples. As shown in

Table 4. Assessment of Calcium content in some baby-food products and pharmaceutical formulations.

Sample	Labeled Amount mg/tab. or mg/g	Potentiometry		AAS		bF-Test
		mg/tab. or Cap.	a Mean% ± SD	mg/tab. or Cap.	a Mean% ± SD
Pharmaceutical formulations
Calcimate (ADWIC, Egypt)	500 mg/tablet	497.3 ± 1.2	99.4 ± 0.7	499.2 ± 0.2	99.8 ± 0.1	4.46
Vitacid calcium (CID, Egypt)	800 mg/tablet	787.3 ± 3.1	98.4 ± 1.1	795.1 ± 0.7	99.4 ± 0.2	3.72
Baby-food products
Cerelac + rice	4.3 mg/g	3.9 ± 0.3	90.7 ± 1.2	4.1 ± 0.5	95.3 ± 2.1	1.76
Cerelac + wheat + 4 fruits	5.0 mg/g	4.7 ± 0.6	94 ± 2.2	5.04 ± 0.4	100.8 ± 0.4	2.23
Nestle® NIDO® FORTIFIED Milk Powder Tin 900 g	8.6 mg/g	8.8 ± 0.4	102.3 ± 0.4	8.7 ± 0.2	101.1 ± 0.7	3.34

^a Mean of three replicate measurements ± standard deviation (SD); ^b F-test at 95% confidence level values is 6.39.

, the data obtained using the proposed sensors are in good agreement with those obtained from AAS measurements [40] for both baby-food and pharmaceutical formulation samples. An F-test showed no observable difference between means and variances of the sets of results obtained by the two methods at 95% confidence level. The F-values calculated (n = 6) were found to be in the range of 2.51–5.15 compared with the tabulated value (6.39) at the 95% confidence level.

The sensors were successfully applied for calcium ion determination in human blood as a biological fluid, where blood samples were collected from different volunteers and the results are listed in

Table 5. Of calcium in human serum samples using the proposed electrodes and the AAS reference method.

Sample	* Calcium Content, mg/dL		aF-Test
	Potentiometry	AAS
Volunteer 1	9.3 ± 1.5	9.5 ± 0.7	4.12
Volunteer 2	10.5 ± 0.9	10.3 ± 0.3	3.36
Volunteer 3	7.8 ± 0.6	8.4 ± 0.2	1.43

* Mean of three replicate measurements ± standard deviation (SD); ^a F-test at 95% confidence level values is 6.39.

. F-test showed no observable difference between means and variances of the two sets of results obtained by the methods at the 95% confidence level. The F-values calculated (n = 6) were found to be in the range of 1.76–4.46, compared with the tabulated value (6.39) at the 95% confidence level.

4. Conclusions

A new type of solid-contact calcium sensors was fabricated characterized and presented for calcium ion determination. Polypyrrol (PPY) and graphene oxide (GO) have been used as ion-to-electron transducers in screen printed solid-contact Ca²⁺-ISEs. The presence of such layers significantly reduced the membrane resistance and enhanced the short-term potential stability of the presented electrodes. In a 50 mM tris buffer solution of pH 5, the sensors revealed good analytical parameters: near-Nernstian responses for Ca²⁺ ions with slopes of 38.1 ± 0.4 (r² = 0.996) and 31.1 ± 0.6 (r² = 0.999) mV/decade, detection limits of 3.8 × 10⁻⁶(0.152 μg/mL) and 2.3 × 10⁻⁷ M (8.0 ng/mL), and linear concentration ranges of 7.0 × 10⁻⁶–1.0 × 10⁻² (400–0.28 μg/mL) and 7.0 × 10⁻⁷–1.0 × 10⁻² M (400–0.028 μg/mL) for sensors based on PPY and GO, respectively. These reliable and robust sensors were successfully applied for calcium ion determination in different baby-food products, pharmaceutical formulations, and human blood samples. The results obtained compared favorably with data collected using atomic absorption spectrometry (AAS).