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Chemosensors 2018, 6(4), 58; https://doi.org/10.3390/chemosensors6040058

Propylene Glycol Stabilizes the Linear Response of Glutamate Biosensor: Potential Implications for In-Vivo Neurochemical Monitoring

1
Department of Medical, Surgical and Experimental Sciences, Sassari University, Viale San Pietro 43/b, 07100 Sassari, Italy
2
Mediterranean Center for Disease Control, Sassari University, Via Vienna 2, 07100 Sassari, Italy
*
Author to whom correspondence should be addressed.
Received: 26 October 2018 / Revised: 22 November 2018 / Accepted: 26 November 2018 / Published: 28 November 2018
(This article belongs to the Special Issue Enzyme-Based Sensing Approaches)
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Abstract

L-glutamate is one the most important excitatory neurotransmitter at the central nervous system level and it is implicated in several pathologies. So, it is very important to monitor its variations, in real time in animal models’ brain. The present study aimed to develop and characterize a new amperometric glutamate biosensor design that exploits the selectivity of Glutamate Oxidase (GluOx) for l-glutamate, and the capability of a small molecule as propylene glycol (PG), never used before, to influence and extend the stability and the activity of enzyme. Different designs were evaluated by modifying the main components in their concentrations to find the most suitable design. Moreover, enzyme concentrations from 100 U/mL up to 200 U/mL were verified and different PG concentrations (1%, 0.1% and 0.05%) were tested. The most suitable selected design was Ptc/PPD/PEI(1%)2/GlutOx5/PG(0.1%) and it was compared to the same already described design loading PEDGE, instead of PG, in terms of over-time performances. The PG has proved to be capable of determining an over-time stability of the glutamate biosensor in particular in terms of linear region slope (LRS) up to 21 days. View Full-Text
Keywords: L-gutamate; amperometric biosensors; propylene glycol; stability over time L-gutamate; amperometric biosensors; propylene glycol; stability over time
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Rocchitta, G.; Bacciu, A.; Arrigo, P.; Migheli, R.; Bazzu, G.; Serra, P.A. Propylene Glycol Stabilizes the Linear Response of Glutamate Biosensor: Potential Implications for In-Vivo Neurochemical Monitoring. Chemosensors 2018, 6, 58.

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