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Article

Virucidal Activity of Different Mouthwashes Using a Novel Biochemical Assay

1
Independent Researcher, 35004 Gijón, Spain
2
Department of Periodontics, AME University Monterrey, Monterrey 64060, Mexico
3
School of Basic and Biomedical Sciences, Universidad Simón Bolívar, Barranquilla 080002, Colombia
4
Department of Biomedical, Surgical and Dental Sciences, University of Milan, 20122 Milan, Italy
5
Department of Periodontics and Oral Medicine, School of Dentistry, University of Michigan, Ann Arbor, MI 48109, USA
*
Authors to whom correspondence should be addressed.
Academic Editor: Daniela Carmagnola
Healthcare 2022, 10(1), 63; https://doi.org/10.3390/healthcare10010063
Received: 15 November 2021 / Revised: 6 December 2021 / Accepted: 21 December 2021 / Published: 30 December 2021
(This article belongs to the Special Issue Oral Healthcare and Prevention during the Pandemic)
Background: Saliva of patients with COVID-19 has a high SARS-CoV-2 viral load. The risk of spreading the virus is not insignificant, and procedures for reducing viral loads in the oral cavity have been proposed. Little research to date has been performed on the effect of mouthwashes on the SARS-CoV-2 virus, and some of their mechanisms of action remain unknown. Methods: SARS-CoV-2 positive nasopharyngeal swabs measured by RT-PCR were used for virucidal activity in a 1:1 ratio, with an incubation time of 1 min. The solutions used in this study were: iodopovidone (8 mg); * D-limonene, a terpene extracted from citrus peels (0.3%); cetylpyridinium chloride (0.1%) (CPC); chlorhexidine gluconate (10%) (CHX); § a CPC (0.12%) and CHX (0.05%) containing formula; ** a formula containing essential oils; †† a CPC containing formula (0.07%); ‡‡ a D-limonene (0.2%) and CPC (0.05%) containing formula; §§ a solution containing sodium fluoride (0.05%) and CPC (0.075%); *** a solution containing CHX (0.12%) and; ††† a CHX (0.2%) containing formula. ‡‡‡ As a control reaction, saline solution or excipient solution (water, glycerin, citric acid, colorant, sodium citrate) was used. Conclusion: Within the limitations of this study, we can conclude that a mouthwash containing both D-limonene and CPC reduced the virucidal activity in about 6 logs (>99.999% reduction). Hence, establishing a clinical protocol for dentists is suggested, where all patients to be treated rinse pre-operatively with a mouthwash containing both D-limonene and CPC to reduce the likelihood of infection with SARS-CoV-2 for dentists. This is a relatively inexpensive way to reduce viral transmission of SARS-CoV-2 from infected individuals within the community. It is also a simple way to decrease infections from asymptomatic and pre-symptomatic patients. View Full-Text
Keywords: mouthwash; SARS-CoV-2; virucidal activity; viral load; COVID-19; CPC; D-limonene; antiseptic mouthwash; SARS-CoV-2; virucidal activity; viral load; COVID-19; CPC; D-limonene; antiseptic
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MDPI and ACS Style

Rodríguez-Casanovas, H.J.; la Rosa, M.D.; Bello-Lemus, Y.; Rasperini, G.; Acosta-Hoyos, A.J. Virucidal Activity of Different Mouthwashes Using a Novel Biochemical Assay. Healthcare 2022, 10, 63. https://doi.org/10.3390/healthcare10010063

AMA Style

Rodríguez-Casanovas HJ, la Rosa MD, Bello-Lemus Y, Rasperini G, Acosta-Hoyos AJ. Virucidal Activity of Different Mouthwashes Using a Novel Biochemical Assay. Healthcare. 2022; 10(1):63. https://doi.org/10.3390/healthcare10010063

Chicago/Turabian Style

Rodríguez-Casanovas, Héctor J., Manuel D. la Rosa, Yesit Bello-Lemus, Giulio Rasperini, and Antonio J. Acosta-Hoyos. 2022. "Virucidal Activity of Different Mouthwashes Using a Novel Biochemical Assay" Healthcare 10, no. 1: 63. https://doi.org/10.3390/healthcare10010063

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