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Fractional Synthesis Rates of Individual Proteins in Rat Soleus and Plantaris Muscles

1
Research Institute for Sport & Exercise Sciences, Liverpool John Moores University, Liverpool L3 3AF, UK
2
Department of Applied Mathematics, Liverpool John Moores University, Liverpool L3 3AF, UK
*
Author to whom correspondence should be addressed.
Proteomes 2020, 8(2), 10; https://doi.org/10.3390/proteomes8020010
Received: 2 April 2020 / Revised: 8 May 2020 / Accepted: 9 May 2020 / Published: 11 May 2020
Differences in the protein composition of fast- and slow-twitch muscle may be maintained by different rates of protein turnover. We investigated protein turnover rates in slow-twitch soleus and fast-twitch plantaris of male Wistar rats (body weight 412 ± 69 g). Animals were assigned to four groups (n = 3, in each), including a control group (0 d) and three groups that received deuterium oxide (D2O) for either 10 days, 20 days or 30 days. D2O administration was initiated by an intraperitoneal injection of 20 μL of 99% D2O-saline per g body weight, and maintained by provision of 4% (v/v) D2O in the drinking water available ad libitum. Soluble proteins from harvested muscles were analysed by liquid chromatography–tandem mass spectrometry and identified against the SwissProt database. The enrichment of D2O and rate constant (k) of protein synthesis was calculated from the abundance of peptide mass isotopomers. The fractional synthesis rate (FSR) of 44 proteins in soleus and 34 proteins in plantaris spanned from 0.58%/day (CO1A1: Collagen alpha-1 chain) to 5.40%/day NDRG2 (N-myc downstream-regulated gene 2 protein). Eight out of 18 proteins identified in both muscles had a different FSR in soleus than in plantaris (p < 0.05). View Full-Text
Keywords: deuterium oxide; fractional synthesis rate; stable isotope labelling; skeletal muscle; protein synthesis; protein turnover; dynamic proteome profiling deuterium oxide; fractional synthesis rate; stable isotope labelling; skeletal muscle; protein synthesis; protein turnover; dynamic proteome profiling
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MDPI and ACS Style

Stead, C.A.; Hesketh, S.J.; Bennett, S.; Sutherland, H.; Jarvis, J.C.; Lisboa, P.J.; Burniston, J.G. Fractional Synthesis Rates of Individual Proteins in Rat Soleus and Plantaris Muscles. Proteomes 2020, 8, 10.

AMA Style

Stead CA, Hesketh SJ, Bennett S, Sutherland H, Jarvis JC, Lisboa PJ, Burniston JG. Fractional Synthesis Rates of Individual Proteins in Rat Soleus and Plantaris Muscles. Proteomes. 2020; 8(2):10.

Chicago/Turabian Style

Stead, Connor A.; Hesketh, Stuart J.; Bennett, Samuel; Sutherland, Hazel; Jarvis, Jonathan C.; Lisboa, Paulo J.; Burniston, Jatin G. 2020. "Fractional Synthesis Rates of Individual Proteins in Rat Soleus and Plantaris Muscles" Proteomes 8, no. 2: 10.

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