Next Article in Journal
Calreticulin as A Novel Potential Metastasis-Associated Protein in Myxoid Liposarcoma, as Revealed by Two-Dimensional Difference Gel Electrophoresis
Next Article in Special Issue
Editorial for Special Issue: Neuroproteomics
Previous Article in Journal
Terminomics Methodologies and the Completeness of Reductive Dimethylation: A Meta-Analysis of Publicly Available Datasets
Previous Article in Special Issue
Correction: Baucum II, Anthony J. et al. Proteomic Analysis of the Spinophilin Interactome in Rodent Striatum Following Psychostimulant Sensitization. Proteomes 2018, 6, 53
Open AccessArticle

Development of Targeted Mass Spectrometry-Based Approaches for Quantitation of Proteins Enriched in the Postsynaptic Density (PSD)

1
Yale/NIDA Neuroproteomics Center, New Haven, CT 06511, USA
2
W.M Keck Biotechnology Resource Laboratory, Yale University School of Medicine, New Haven, CT 06511, USA
3
Molecular Biophysics and Biochemistry, Yale University School of Medicine, New Haven, CT 06511, USA
4
Tanvex BioPharma Inc., San Diego, CA 92121, USA
5
Department of Neurology and Neurological Science, Tokyo Medical and Dental University, Tokyo 113-8519, Japan
6
Department of Psychiatry, Yale School of Medicine, Connecticut Mental Health Center, New Haven, CT 06511, USA
*
Author to whom correspondence should be addressed.
Proteomes 2019, 7(2), 12; https://doi.org/10.3390/proteomes7020012
Received: 11 March 2019 / Revised: 27 March 2019 / Accepted: 28 March 2019 / Published: 2 April 2019
(This article belongs to the Special Issue Neuroproteomics)
The postsynaptic density (PSD) is a structural, electron-dense region of excitatory glutamatergic synapses, which is involved in a variety of cellular and signaling processes in neurons. The PSD is comprised of a large network of proteins, many of which have been implicated in a wide variety of neuropsychiatric disorders. Biochemical fractionation combined with mass spectrometry analyses have enabled an in-depth understanding of the protein composition of the PSD. However, the PSD composition may change rapidly in response to stimuli, and robust and reproducible methods to thoroughly quantify changes in protein abundance are warranted. Here, we report on the development of two types of targeted mass spectrometry-based assays for quantitation of PSD-enriched proteins. In total, we quantified 50 PSD proteins in a targeted, parallel reaction monitoring (PRM) assay using heavy-labeled, synthetic internal peptide standards and identified and quantified over 2100 proteins through a pre-determined spectral library using a data-independent acquisition (DIA) approach in PSD fractions isolated from mouse cortical brain tissue. View Full-Text
Keywords: postsynaptic density; PSD; parallel reaction monitoring; PRM; targeted proteomics; data-independent acquisition; DIA; quantitative mass spectrometry postsynaptic density; PSD; parallel reaction monitoring; PRM; targeted proteomics; data-independent acquisition; DIA; quantitative mass spectrometry
Show Figures

Figure 1

MDPI and ACS Style

Wilson, R.S.; Rauniyar, N.; Sakaue, F.; Lam, T.T.; Williams, K.R.; Nairn, A.C. Development of Targeted Mass Spectrometry-Based Approaches for Quantitation of Proteins Enriched in the Postsynaptic Density (PSD). Proteomes 2019, 7, 12.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop