Next Article in Journal
Development of Targeted Mass Spectrometry-Based Approaches for Quantitation of Proteins Enriched in the Postsynaptic Density (PSD)
Previous Article in Journal
Comparative Proteomic Analysis Unveils Critical Pathways Underlying the Role of Nitrogen Fertilizer Treatment in American Elderberry
 
 
Search for Articles:
Title / Keyword
Author / Affiliation
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
Proteomes
Volume 7
Issue 2
10.3390/proteomes7020011
proteomes-logo
Submit to this Journal Review for this Journal Edit a Special Issue
► Article Menu

Article Menu

share announcement question_answer thumb_up
...
textsms
...
Technical Note

Terminomics Methodologies and the Completeness of Reductive Dimethylation: A Meta-Analysis of Publicly Available Datasets

by
Mariella Hurtado Silva
1,
Iain J. Berry
1,2,
Natalie Strange
1,
Steven P. Djordjevic
2 and
Matthew P. Padula
1,*
1
Proteomics Core Facility and School of Life Sciences, Faculty of Science, University of Technology Sydney, Broadway NSW 2007, Australia
2
The ithree Institute, Faculty of Science, University of Technology Sydney, Broadway NSW 2007, Australia
*
Author to whom correspondence should be addressed.
Proteomes 2019, 7(2), 11; https://doi.org/10.3390/proteomes7020011
Received: 16 January 2019 / Revised: 22 March 2019 / Accepted: 25 March 2019 / Published: 29 March 2019
View Full-Text Download PDF
Browse Figures
Review Reports

Abstract

Methods for analyzing the terminal sequences of proteins have been refined over the previous decade; however, few studies have evaluated the quality of the data that have been produced from those methodologies. While performing global N-terminal labelling on bacteria, we observed that the labelling was not complete and investigated whether this was a common occurrence. We assessed the completeness of labelling in a selection of existing, publicly available N-terminomics datasets and empirically determined that amine-based labelling chemistry does not achieve complete labelling and potentially has issues with labelling amine groups at sequence-specific residues. This finding led us to conduct a thorough review of the historical literature that showed that this is not an unexpected finding, with numerous publications reporting incomplete labelling. These findings have implications for the quantitation of N-terminal peptides and the biological interpretations of these data. View Full-Text
Keywords: terminomics; mass spectrometry; amine labelling terminomics; mass spectrometry; amine labelling
►▼ Show Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

SciFeed

Share and Cite

MDPI and ACS Style

Hurtado Silva, M.; Berry, I.J.; Strange, N.; Djordjevic, S.P.; Padula, M.P. Terminomics Methodologies and the Completeness of Reductive Dimethylation: A Meta-Analysis of Publicly Available Datasets. Proteomes 2019, 7, 11. https://doi.org/10.3390/proteomes7020011

AMA Style

Hurtado Silva M, Berry IJ, Strange N, Djordjevic SP, Padula MP. Terminomics Methodologies and the Completeness of Reductive Dimethylation: A Meta-Analysis of Publicly Available Datasets. Proteomes. 2019; 7(2):11. https://doi.org/10.3390/proteomes7020011

Chicago/Turabian Style

Hurtado Silva, Mariella, Iain J. Berry, Natalie Strange, Steven P. Djordjevic, and Matthew P. Padula. 2019. "Terminomics Methodologies and the Completeness of Reductive Dimethylation: A Meta-Analysis of Publicly Available Datasets" Proteomes 7, no. 2: 11. https://doi.org/10.3390/proteomes7020011

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Metrics

Article Access Map by Country/Region

1
Back to TopTop