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A Proteomic View of Salmonella Typhimurium in Response to Phosphate Limitation

Institute of Analytical Chemistry and Synthetic and Functional Biomolecules Center, College of Chemistry and Molecular Engineering, Peking University, 202 Chengfu Rd, Haidian District, Beijing 100871, China
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Proteomes 2018, 6(2), 19; https://doi.org/10.3390/proteomes6020019
Received: 25 March 2018 / Revised: 22 April 2018 / Accepted: 23 April 2018 / Published: 25 April 2018
Salmonella enterica serovar Typhimurium (S. Typhimurium), an important foodborne pathogen, often encounters phosphate (Pi) shortage both in the environment and inside host cells. To gain a global view on its physiological responses to Pi starvation, we performed proteomic profiling of S. Typhimurium upon the shift from Pi-rich to Pi-low conditions. In addition to the Pho regulon, many metabolic processes were up-regulated, such as glycolysis, pentose phosphate pathway, pyrimidine degradation, glycogen, and trehalose metabolism, allowing us to chart an overview of S. Typhimurium carbon metabolism under Pi starvation. Furthermore, proteomic analysis of a mutant lacking phoB (that encodes a key regulator of Pi shortage response) suggested that only a small subset of the altered proteins upon Pi limitation was PhoB-dependent. Importantly, we present evidence that S. Typhimurium N-acetylglucosamine catabolism was induced under Pi-limiting conditions in a PhoB-dependent manner. Immunoblotting and β-galactosidase assays demonstrated that PhoB was required for the full activation of NagB, a key enzyme of this pathway, in response to low Pi. Thus, our study reveals that N-acetylglucosamine catabolism may represent an additional PhoB-regulated pathway to tackle bacterial Pi shortage. View Full-Text
Keywords: Salmonella Typhimurium; phosphate starvation; the PhoR-PhoB two-component system; the nag operon Salmonella Typhimurium; phosphate starvation; the PhoR-PhoB two-component system; the nag operon
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Jiang, J.; Yu, K.; Qi, L.; Liu, Y.; Cheng, S.; Wu, M.; Wang, Z.; Fu, J.; Liu, X. A Proteomic View of Salmonella Typhimurium in Response to Phosphate Limitation. Proteomes 2018, 6, 19.

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