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Isoelectric Point Separations of Peptides and Proteins

Department of Chemistry, University of Illinois at Chicago, Chicago, IL 60607, USA
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Author to whom correspondence should be addressed.
Academic Editors: Jens R. Coorssen, Alfred L. Yergey and Jacek R. Wisniewski
Proteomes 2017, 5(1), 4; https://doi.org/10.3390/proteomes5010004
Received: 22 October 2016 / Revised: 3 January 2017 / Accepted: 8 January 2017 / Published: 25 January 2017
The separation of ampholytic components according to isoelectric point has played an important role in isolating, reducing complexity and improving peptide and protein detection. This brief review outlines the basics of isoelectric focusing, including a summary of the historical achievements and considerations in experimental design. Derivative methodologies of isoelectric focusing are also discussed including common detection methods used. Applications in a variety of fields using isoelectric point based separations are provided as well as an outlook on the field for future studies. View Full-Text
Keywords: isoelectric focusing; proteomics; electrophoresis; two-dimensional gel electrophoresis; isoelectric trapping; capillary isoelectric focusing isoelectric focusing; proteomics; electrophoresis; two-dimensional gel electrophoresis; isoelectric trapping; capillary isoelectric focusing
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MDPI and ACS Style

Pergande, M.R.; Cologna, S.M. Isoelectric Point Separations of Peptides and Proteins. Proteomes 2017, 5, 4.

AMA Style

Pergande MR, Cologna SM. Isoelectric Point Separations of Peptides and Proteins. Proteomes. 2017; 5(1):4.

Chicago/Turabian Style

Pergande, Melissa R.; Cologna, Stephanie M. 2017. "Isoelectric Point Separations of Peptides and Proteins" Proteomes 5, no. 1: 4.

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