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Open AccessArticle

Targeted Enlargement of Aptamer Functionalized Gold Nanoparticles for Quantitative Protein Analysis

Department of Laboratory Medicine and Pathology, University of Alberta, Edmonton, AB T6G 2G3, Canada
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Authors to whom correspondence should be addressed.
Current Address: Department of Chemistry, Brock University, St. Catharines, ON L2S 3A1, Canada.
Academic Editors: Jens R. Coorssen, Alfred L. Yergey and Jacek R. Wisniewski
Proteomes 2017, 5(1), 1; https://doi.org/10.3390/proteomes5010001
Received: 1 November 2016 / Revised: 12 December 2016 / Accepted: 18 December 2016 / Published: 22 December 2016
The ability to selectively amplify the detection signals for targets over interferences is crucial when analyzing proteins in a complicated sample matrix. Here, we describe a targeted enlargement strategy that can amplify the light-scattering signal from aptamer-functionalized gold nanoparticles (Apt-AuNP) with high specificity for quantitative protein analysis. This strategy is achieved by labeling target proteins with competitively protected Apt-AuNP probes and enlarging the probes with gold enhancement. This competitive protection strategy could effectively eliminate nonspecific protein adsorptions from a sample matrix, leading to a highly specific labeling of the target protein. As a result, the subsequent amplification of the light-scattering signal by gold enhancement only occurs in the presence of the target protein. This strategy was successfully demonstrated by analyzing human α-thrombin in human serum samples in a Western blot format. View Full-Text
Keywords: aptamer; protein quantification; Western blot; gold enlargement aptamer; protein quantification; Western blot; gold enlargement
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MDPI and ACS Style

Li, F.; Li, J.; Tang, Y.; Wang, C.; Li, X.-F.; Le, X.C. Targeted Enlargement of Aptamer Functionalized Gold Nanoparticles for Quantitative Protein Analysis. Proteomes 2017, 5, 1. https://doi.org/10.3390/proteomes5010001

AMA Style

Li F, Li J, Tang Y, Wang C, Li X-F, Le XC. Targeted Enlargement of Aptamer Functionalized Gold Nanoparticles for Quantitative Protein Analysis. Proteomes. 2017; 5(1):1. https://doi.org/10.3390/proteomes5010001

Chicago/Turabian Style

Li, Feng; Li, Jingjing; Tang, Yanan; Wang, Chuan; Li, Xing-Fang; Le, X. C. 2017. "Targeted Enlargement of Aptamer Functionalized Gold Nanoparticles for Quantitative Protein Analysis" Proteomes 5, no. 1: 1. https://doi.org/10.3390/proteomes5010001

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