Measurement Error of Markerless Motion Capture Systems Applied to Tracking Movements in Human–Object Interaction Tasks: A Systematic Review with Best Evidence Synthesis

This systematic review focused on the validity of markerless motion capture (MMC) systems used for human movement assessment during tasks that involve physical interaction with objects. Five electronic databases were searched until May 2025. Eligible studies (i) assessed the validity of an MMC system, (ii) required human participants to perform tasks that involved physical interaction with objects (e.g., lifts, carrying, gait with loads), (iii) employed a marker-based reference system, and (iv) reported at least one kinematic metric. Risk of bias was assessed using the SURE checklist. A best-evidence synthesis was conducted to classify the level of evidence across included studies. Fifteen studies met eligibility (median = 21 participants per study). In general, MMC systems presented good performance in capturing the waveforms related to movement (i.e., high associations with reference systems), but its level of precision (i.e., the magnitude of differences to the reference systems) still requires improvement regarding tasks involving human–object interactions. Most tasks analyzed were lifts, gait with load, squatting and reaching/manipulation, and technical gestures. There was strong evidence for the validity of MMC for implementation during lifting tasks. In summary, markerless motion capture (MMC) systems exhibit promising evidence of validity for some human–object interaction tasks, that is, especially when lifting as strong evidence was observed across studies on this type of task. In contrast, some evidence for tasks including gait under load, squatting, reaching, or touchscreen interaction is limited, moderate, or conflicting. Notwithstanding these limitations, most studies were observed to have moderate- to high-quality methodology. Additional research is required to optimize protocols to study the measurement error aspects of MMC under human–object interaction in real-world environments.